Figure 2
From: Lemon protein disulfide isomerase: cDNA cloning and biochemical characterization
Expression and purification of recombinant ClPDI in E. coli. Fifteen μL (loading buffer without mercaptoethanol and without boiling) of each fraction was loaded into each lane of the 10% SDS-PAGE followed by Coomassie Brilliant Blue R-250 staining. Lane 1, crude extract from E. coli expressing ClPDI; 2, flow-through proteins from the Ni-NTA column; 3, wash; 4–7, ClPDI eluted from Ni-NTA column. Molecular masses (in kDa) of standards are shown at left.