Aspidistra crassifila (Asparagaceae), a new species from Guangxi, China
© Lin et al.; licensee Springer. 2013
Received: 5 March 2013
Accepted: 11 April 2013
Published: 7 October 2013
Aspidistra crassifila Yan Liu & C.-I Peng, a new species of the Asparagaceae from Guangxi Zhuang Autonomous Region, China, is described and illustrated.
The new species is similar to A. subrotata Y. Wan & C. C. Huang in the perianth lobes triangular-lanceolate and horizontally spreading, but differs by the perianth campanulate, lobes with appendages at base, stamens 6–8 mm long, filaments enlarged, anthers adnate to perianth tube, connectives extended and upcurved. The chromosome number of the new species was determined to be 2n = 38, and the karyotype was formulated as 2n = 22m2SC+4sm+12st.
A careful study of the literature, herbarium specimens and living plants, both in the wild and in cultivation in the experimental greenhouse, support the recognition of the new species Aspidistra crassifila, which is described herein. Aspidistra crassifila is currently known only from Shiwandashan Mountains, which lie in southern Guangxi. A line drawing, color plates and a distribution map are given for the new species to aid in identification.
KeywordsAspidistra crassifila Aspidistra subrotata Asparagaceae New species Chromosome number Karyotype Guangxi China
The genus Aspidistra Ker-Gawler (Asparagaceae) was established in 1822 and is comprised of ca. 100 species, more than 60 of which occur in China (Lang et al., 1999; Li, 2004; Tillich, 20052008; Hou et al., 2009; Lin et al., 20092010; Liu et al., 2011). In March 2005, during a field trip to Shiwandashan Mountains in southern Guangxi Zhuang Autonomous Region, China, the second author (Yan Liu) collected and brought back a sterile plant of Aspidistra for cultivation, which flowered in Guilin Botanical Garden next spring. We went on another trip to the same locality in Shiwandashan Mountains in January 2007 and were able to collect fruiting materials of this species. Compared with other species of Aspidistra, it was recognized as an undescribed species that differs from congeners in its peculiar adnate stamens with extended and upcurved connectives.
Somatic chromosomes were examined for plants collected from the type collection (Yan Liu L1380). Root tips were pretreated in 2 mM 8-hydroxyquinoline at 15-18°C for about 8 h, then fixed overnight in a 3:1 ethanol-acetic acid solution below 4°C. The chromosomes were stained with 2% acetic orcein in 1mol/L hydrochloric acid and observed. Classification of chromosome morphology is based on the position of the centromere, following Levan et al. (1964).
Results and discussion
Species nova A. subrotatae Y. Wan & C. C. Huang affinis, sed differt perianthio campanulato (vs. subrotato), lobis basi appendiculatis (vs. non appendiculatis, margine reflexis), filamentis dilatatis (vs. non dilatatis), antheris adnatis (vs. versatilibus), connectivis supra antheras productis (vs. non productis).
Herbs perennial, evergreen, rhizomatous. Rhizome creeping, subterete, 8–13 mm thick, covered with scales, nodes dense. Vaginal leaves 4–5, 1–14 cm long, purple-red, enveloping base of petiole, fibrous when withered. Leaves solitary, 2–5 cm apart; petiole stiff , upright, 10–40 cm long, 3–5 mm thick, adaxially sulcate; leaf blade oblong-oblanceolate, 30–60 cm long, 6–12 cm wide, base cuneate, gradually narrowed into petiole, inequilateral, apex acuminate, margin entire. Peduncle purplish red to purplish black, 2.5-6 cm long, with 4–5 bracts, bracts gradually wider from base to top of peduncle; the uppermost bract broadly ovate, purplish red or purplish black, ca. 10 mm long, ca. 12 mm wide, apex subobtuse. Flowers solitary; perianth purplish black, fleshy, campanulate, 4–6 cm in diam., 8–12 lobed apically; lobes triangular-lanceolate, 15–25 mm long and 5–10 mm wide at base, apex gradually acuminate, horizontally spreading, with appendages at base, tube 8–12 mm long, distal opening 15–20 mm diam.; stamens as many as and opposite to lobes, 6–8 mm long, inserted in the middle of perianth tube, positioned lower than stigma, filaments purplish black, enlarged, 3–4 mm wide at side view, their upper surfaces visible from above (between adaxial surface of perianth tube and margin of stigma), anthers adnate to perianth, pale yellow, oblong, 5–6 mm long, ca. 2 mm wide, connectives extended and upcurved; pistil mushroom-shaped, 2 cm long, style cylindrical, ca. 10 mm long, ca. 4 mm across, purplish red, ovary inconspicuous, stigma enlarged, ca. 10 mm high, 12–15 mm across, upper surface white with purple spots, smooth, lower surface purplish black, irregularly concaved and undulate at margin. Berry subglobose, ca. 3.5 cm across, tuberculate.
Additional specimens examined
CHINA. Guangxi Zhuang Autonomous Region, Fangcheng (City), Shiwandashan Mountains, alt. 980 m, 10 March 2005, Yan Liu L1156 (IBK); same locality, 17 January 2007, Yan Liu L1425 (IBK).
Previously, detailed cytological data were known for 42 species in the genus Aspidistra (Bogner and Arnautov, 2004; Li, 2004; Yamashita and Tamura, 2004; Qiao et al., 2008; Hou et al., 2009, Lin et al., 2010; Liu et al., 2011). Aspidistra crassifila shared a number of cytological features, namely basic chromosome numbers of x = 19, trimodal chromosome complement, the first pair of longer median chromosomes and 10th median SC-chromosomes, in common with those of the Aspidistra species previously reported (Lin et al., 2010).
On shaded bamboo slopes in seasonal rain forests (monsoon forests).
Flowering from March to May; fruits maturing in May next year.
The specific epithet 'crassifila' is derived from its enlarged filaments.
A careful study of the literature, herbarium specimens and living plants, both in the wild and in cultivation in the experimental greenhouse, support the recognition of the new species Aspidistra crassifila, which is described herein. Aspidistra crassifila is currently known only from Shiwandashan Mountains, which lie in southern Guangxi.
The authors are grateful to Prof. Fa-Nan Wei (IBK) for Latin diagnosis. We also thank Mr. Shun-Qing He (IBK) for the handsome drawing. This study was supported by the Guangxi Natural Science Foundation (2010GXNSFE013004, 2011GXNSFE18001) to Yan Liu; postdoctoral fellowship from Academia Sinica, Taiwan to Yoshiko Kono; and National Geographic Society Grant # 8358–07 (Botanical Exploration of Limestone Karsts of Southern Guangxi, China) to Ching-I Peng.
- Bogner J, Arnautov N: Aspidistra locii (Convallariaceae), an unusual new species from Vietnam. Willdenowia 2004, 34: 203–208. 10.3372/wi.34.34119View ArticleGoogle Scholar
- Bräuchler C, Ngoc LH: Aspidistra renatae (Ruscaceae), a new species from central Vietnam. Blumea 2005, 50: 527–529. 10.3767/000651905X622798View ArticleGoogle Scholar
- Fang D, Yu L-Y: Three new species of Aspidistra Ker-Gawl. (Liliaceae) from Guangxi, China. Acta Phytotax. Sin 2002, 40: 159–163.Google Scholar
- Hou M-F, Liu Y, Kono Y, Peng C-I: Aspidistra daxinensis (Ruscaceae), a new species from limestone areas in Guangxi. China. Bot. Stud 2009, 50: 371–378.Google Scholar
- Lang K-Y, Li G-Z, Liu Y, Wei Y-G, Wang R-X: Taxonomic and phytogeographic studies on the genus Aspidistra Ker-Gawl. (Liliaceae) in China. Acta Phytotax. Sin 1999, 37: 468–508.Google Scholar
- Levan A, Fredga K, Sandberg AA: Nomenclature for centromeric position on chromosomes. Hereditas 1964, 52: 201–220.View ArticleGoogle Scholar
- Li G-Z: The Genus Aspidistra. Nanning: Guangxi Science & Technology Publishing House; 2004.Google Scholar
- Lin C-R, Liang Y-Y, Liu Y: Aspidistra bamaensis (Ruscaceae), a new species from Guangxi. China. Ann. Bot. Fenn 2009, 46: 416–418. 10.5735/085.046.0506View ArticleGoogle Scholar
- Lin C-R, Peng C-I, Kono Y, Liu Y: Aspidistra obconica , Asparagaceae [Ruscaceae], a new species from limestone areas in Guangxi. China. Bot. Stud 2010, 51: 263–268.Google Scholar
- Liu Y, Kono Y, Lin C-R, Xu W-B, Peng C-I: Aspidistra erecta (Asparagaceae), a new species from limestone areas in Guangxi. China. Bot. Stud 2011, 52: 367–373.Google Scholar
- Qiao Q, Zhang C-Q, Ma Y-P, Tian W: Karyotype asymmetry of Aspidistra (Convallarieae, Ruscaceae). Acta Bot. Yunnan 2008, 30: 565–569.Google Scholar
- Tillich HJ: A key for Aspidistra (Ruscaceae), including fifteen new species from Vietnam. Feddes Repert 2005, 116: 313–338. 10.1002/fedr.200511076View ArticleGoogle Scholar
- Tillich HJ: An updated and improved determination key for Aspidistra Ker-Gawl. (Ruscaceae, Monocotyledons). Feddes Repert 2008, 119: 449–462. 10.1002/fedr.200811174View ArticleGoogle Scholar
- Tillich HJ, Averyanov LV: Two new species and one new subspecies of Aspidistra Ker-Gawl. (Ruscaceae) from Vietnam. Feddes Repert 2008, 119: 37–41. 10.1002/fedr.200711143View ArticleGoogle Scholar
- Wan Y, Huang C-C: New species of the genus Aspidistra from Guangxi. Guihaia 1987, 7: 217–224.Google Scholar
- Yamashita J, Tamura MN: Phylogenetic analyses and chromosome evolution in Convallarieae (Ruscaceae sensu lato), with some taxonomic treatments. J Plant Res 2004, 117: 363–370. 10.1007/s10265-004-0169-zView ArticlePubMedGoogle Scholar
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