Figure 3

mBFP was induced as a reporter in different tissue types among plant species under various conditions. A. Tobacco leaves was infiltrated with Agrobacteria carrying mBFP expression constructs driven by DR5 promoter. The infiltrated tissues were further treated with or without 50 μM NAA for 24 hr. Subsequently, the tissues were imaged under fluorescence microscope with UV excitation (UV) or bright light (BL). The induced mBFP proteins were targeted to cytosol (pBin-D-Cy-mBFP), apoplast (pBin-D-Se-mBFP), ER (pBin-D-Er-mBFP), chloroplast (pBin-D-Cp-mBFP) or mitochondria (pBin-D-Mt-mBFP), respectively. Control, Agrobacteria only; +, 50 μM NAA treatment; −, mock treatment. B. The flowers of moth orchid were carried out as the same as described in A. C. The mBFP expression cassettes driven by Adh promoter were transiently expressed in the petals of moth orchid. After agroinfiltration, the inflorescences were treated with or without 1 mM H₂O₂ for 24 hr. The induced mBFP proteins were targeted to cytosol (pBin-A-Cy-mBFP), apoplast (pBin-A-Se-mBFP), ER (pBin-A-Er-mBFP), chloroplast (pBin-A-Cp-mBFP) or mitochondria (pBin-A-Mt-mBFP), respectively. Control, Agrobacteria only; +, 1 mM H₂O₂ treatment; −, mock treatment. D. Five different transgenic Arabidopsis lines in which mBFP gene expression was driven by Adh promoter were generated by floral dip method, and the expressed proteins were targeted to cytosol (pBin-A-Cy-mBFP line), apoplast (pBin-A-Se-mBFP line), ER (pBin-A-Er-mBFP line), chloroplast (pBin-A-Cp-mBFP line) or mitochondria (pBin-A-Mt-mBFP line), respectively. The seeds of Arabidopsis were plated on MS media containing kanamycin, and subsequently were grown vertically (V) or horizontally (H) for 14 days after germination. The regions surrounding the root tips were imaged under fluorescence microscope with UV excitation (UV) or bright light (BL). W, wild type Arabidopsis. The magnification is 400 X (A), 200 X (B, C) or 40 X (D) and the ruler is 20 μm (A), 40 μm (B, C) or 1 mm (D).