Fig. 2

Influence of PGRs and container closure on callus proliferation in Saussurea involucrata Kar. et Kir.: a and f: BA (1.0) + 2,4-D (1.0); b and g: BA (1.0) + 2,4-D (0.5); c and h: BA (2.0) + 2,4-D (0.5); d and i: BA (0.5) + 2,4-D (0.5); e and j: BA (0.5) + NAA (0.5). PGR conc. - mg/L⁻¹; Scale bar for a–j = 1 cm. Culture medium: 1/2X MSBM, 3 % sucrose, 04 % gelrite. Culture tubes in treatments a to e were closed with 2 layers of aluminum foil (2AF), while in treatments f to j were closed with. 3 layers of dispense papers (3DP). Incubation at 20 °C for 4 weeks