Fig. 2

Topology of the proposed SaSTT3A and SaSTT3B in ER membrane. The model was performed using TOPO2 (http://www.sacs.ucsf.edu/TOPO2/) prediction methods. The hydrophobic regions corresponding to transmembrane segments were predicted by the TMpred program (http://www.ch.embnet.org/software/TMPRED_form.html). Putative transmembrane helices (TM) were highlighted in black. SaSTT3A had 13 TM helices (a) while SaSTT3B had 11 (b). NetNGlyc 1.0 Server (http://www.cbs.dtu.dk/services/NetNGlyc/) was used to predict N-glycosylation sites. The locations of putative N-glycosylation sites were shown in larger characters. Both SaSTT3A and SaSTT3B carried out a luminal orientation of the C-terminal domain