Arabidopsis SFAR4 is a novel GDSL-type esterase involved in fatty acid degradation and glucose tolerance
© Huang et al. 2015
Received: 2 September 2015
Accepted: 16 November 2015
Published: 1 December 2015
SFARs (seed fatty acid reducers) belonging to the GDSL lipases/esterases family have been reported to reduce fatty acid storage and composition in mature Arabidopsis seeds. GDSL lipases/esterases are hydrolytic enzymes that possess multifunctional properties, such as broad substrate specificity, regiospecificity, and stereoselectivity. Studies on the physiological functions and biochemical characteristics of GDSL lipases/esterases in plants are limited, so it is important to elucidate the molecular functions of GDSL-type genes.
We found that SFAR4 (At3g48460), a fatty acid reducer belonging to the Arabidopsis GDSL lipases/esterases family, was intensely expressed in embryo protrusion, early seedlings, and pollen. The characterization of recombinant SFAR4 protein indicated that it has short-length p-nitrophenyl esterase activity. In addition, SFAR4 enhanced the expression of genes involved in fatty acid metabolism during seed germination and seedling development. SFAR4 elevated the expression of COMATOSE, which transports fatty acids into peroxisomes, and of LACS6 and LACS7, which deliver long-chain acetyl-CoA for β-oxidation. Furthermore, SFAR4 increased the transcription of PED1 and PNC1, which function in importing peroxisomal ATP required for fatty acid degradation. SFAR4 has another function on tolerance to high glucose concentrations but had no significant effects on the expression of the glucose sensor HXK1.
The results demonstrated that SFAR4 is a GDSL-type esterase involved in fatty acid metabolism during post-germination and seedling development in Arabidopsis. We suggested that SFAR4 plays an important role in fatty acid degradation, thus reducing the fatty acid content.
KeywordsSFAR GDSL Arabidopsis Glucose tolerance
GDSL-type esterases/lipases, a new subclass of lipolytic enzymes, are hydrolytic enzymes with multifunctional properties such as broad substrate specificity, regiospecificity, and stereoselectivity. They are widely distributed in bacteria and plants. GDSL lipolytic enzymes possess a distinct GDSL motif with a serine-containing active site near the N-terminus; hence, they differ from classical lipolytic enzymes with a G × S × G motif near the central conserved domain (Upton and Buckley 1995). Studies have illustrated that GDSL lipolytic enzymes have flexible activities, including thioesterase, protease, arylesterase, and lysophospholipase activities (Akoh et al. 2004; Molgaard et al. 2000; Li et al. 2000; Dalrymple et al. 1997). GDSL-type esterases/lipases have also been detected in various plant species, including Arabidopsis, rice, and maize, and they have been implicated in plant development, morphogenesis, and defense responses (Brick et al. 1995). Several GDSL lipase/esterases in various plant species possess substrate specificity. BnSCE3/BnLIP2 from Brassica napus L. has been reported as a sinapine esterase that strongly expressed during seed germination and that functions in both germination and morphogenesis (Ling et al. 2006; Clauss et al. 2008, 2011). An acetyl-CoA carboxylase gene, AmGDSH1, was cloned in black-grass (Alopecurus myosuroides), and the characterization of the purified protein revealed that it was a carboxyesterase that activated the hydrolysis of the herbicide aryloxyphenoxypropionate (AOPP) (Cummins and Edwards 2004). Pepper CaGLIP1 exhibited various activities, such as the hydrolysis of long-chain and short-chain p-nitrophenyl esters (Hong et al. 2008). A GDSL lipase/esterase isolated from sunflower (Helianthus annuus L.) seeds exhibited fatty acyl-ester hydrolase activity (Beisson et al. 1997). So far, plant GDSL-type esterases/lipases have not been proven to exhibit any lipase activity during seed germination or post-germinative growth or to mediate the relationship between stress and signaling pathway in Arabidopsis.
Gibberellic acid (GA) is essential for numerous aspects of plant development, such as seed germination, leaf expansion, stem elongation, flowering, and seed development (Richards et al. 2001; Sun and Gubler 2004). The DELLA proteins (RGA, GAI, RGL1, RGL2, and RGL3), comprising a class of GA signaling repressors, possess a highly conserved motif in their N-terminal domain in Arabidopsis. The presumptive DELLA-regulated genes involved in seed germination are highly distinct from those involved in flower development. An observation of the transcriptomes of DELLA mutants suggested that GA-mediated seed germination and flower development are distinctly controlled by DELLA-dependent transcriptomes (Cao et al. 2006). Recently, genes encoding several GDSL proteins, named SFAR genes, which are genes downregulated by DELLAs, have been reported to reduce fatty acid storage and composition in mature Arabidopsis seeds and to enhance tolerance to glucose stress during the seed germination (Chen et al. 2012). Among these genes, SFAR4 has been demonstrated to reduce the total fatty acid content and change the composition of unsaturated fatty acids in storage seeds. Transgenic plants with overexpressed SFAR4 exhibited tolerance to glucose stress and a higher germination rate (Chen et al. 2012). The relationship between glucose stress and SFAR genes in germinating seeds or during post-germinative seedling growth remains unknown. The mechanism underlying SFAR4-induced insensitivity to glucose in seeds or seedlings has not yet been elucidated.
Seed germination is associated with the degradation and mobilization of accumulated components in mature seeds (Bewley 1997). During germination, the Arabidopsis storage lipids are converted to sucrose through the glyoxylate cycle. Sucrose synthesis and the peroxisomal pathways are most crucial to post-germinative growth (Penfield et al. 2004; Holdsworth et al. 2008; Footitt et al. 2006). Sucrose stored in vacuoles or converted into glucose or UDP-glucose and fructose is essential as an energy source and for signaling during seed germination (Koch 2004). However, a high sugar concentration delays seed germination and inhibits cotyledon expansion and greening, true leaf formation, and root growth of Arabidopsis seedlings (Rolland et al. 2006). Exogenous glucose significantly retards the mobilization of seed storage lipids in germinating Arabidopsis seeds. Critical metabolic transition of the primary sugar source results in a loss of sensitivity to the inhibitory effect of glucose on lipid breakdown and early seedling development (To et al. 2002). Two genes involved in the glyoxylate cycle and playing a role in lipid metabolism, malate synthase (MS) and isocitrate lyase (ICL), have been reported to be negatively regulated by sugars. Using glyoxylate cycle mutants as a paradigms also reveals that they are resistant to the inhibitory effects of exogenous glucose on storage seed lipid breakdown in germinating seeds (Graham et al. 1994). Products of fatty acid catabolism can pass from the peroxisome to the mitochondrion independently of the glyoxylate cycle (Eastmond et al. 2000). Comprehensive metabolic profiling studies have suggested that Arabidopsis seed germination efficiency is not only affected by the accumulation of reserves during seed development or by their mobilization during seed germination but also by additional, as yet unknown factors (Fait et al. 2006).
Hayashi et al. used ped mutants, which are necessary to maintain glyoxysomal function, to demonstrate that peroxisomal fatty acid β-oxidation plays an important role in producing sucrose from storage lipids during germination (Hayashi et al. 1998). Studies on the deficiency of 3-ketoacyl-CoA thiolase, PED1, have suggested that peroxisomal β-oxidation is essential for post-germinative growth in Arabidopsis (Poirier et al. 1999; Hayashi et al. 1998). During post-germinative growth, peroxisomal β-oxidation of fatty acids releases storage triacylglycerol to produce acetyl-CoA and converts it to sucrose as a source of metabolic energy and carbon skeletons. Prior to β-oxidation, fatty acids are transported into peroxisomes by CTS (Footitt et al. 2002), and acyl-CoA substrates for β-oxidation are delivered by peroxisomal long-chain acyl-CoA synthetases (LACS6 and LACS7) (Fulda et al. 2004). Both steps are required for post-germinative storage oil breakdown for successful seedling establishment. Recently, repressing peroxisomal adenine nucleotide carriers PNC1 and PNC2 were been found to impair peroxisomal ATP import, which inhibits fatty acid breakdown during early seedling growth (Linka et al. 2008). However, those studies indicate that β-oxidation of fatty acids is not required for Arabidopsis germination (Hayashi et al. 1998; Poirier et al. 1999; Fulda et al. 2004). Because fatty acids hydrolyzed from storage lipids are converted to CoA thioesters, storage lipid breakdown plays an important role during germination in oilseed plants (Graham and Eastmond 2002).
Exogenous sucrose delays lipid breakdown in Arabidopsis seedlings (Eastmond et al. 2000), and high sugar concentrations repress seed germination and seedling growth. However, the signal transduction pathways involved in responses to sugar are not fully known. Hexokinase (HXK) signaling may inhibit the mobilization of lipids and other storage compounds, thus preventing germination (Pego et al. 1999; Smeekens 2000). HXK and SNF1-related protein kinases are postulated to control several sugar-regulated processes. Hexokinase 1 (HXK1) is a sugar sensor with both signaling and metabolic functions in plants (Jang et al. 1997). In the sugar signaling pathway, HXK1 is a glucose sensor that modulates gene expression and multiple plant hormone-signaling pathways underlying sugar sensing and signaling in plants (Pego et al. 1999; Jang et al. 1997). Limited information exists regarding the role of GDSL-type genes in determining lipid metabolic cellular signaling during seed germination and post-germinative growth. It therefore becomes important to study the role of GDSL-type genes involved in lipid metabolic pathways and the mechanisms underlying sugar sensitivity.
This study provides evidence that SFAR4 plays a crucial role in the lipid metabolic process and induction of tolerance to glucose stress through a glucose sensor-independent pathway during seed germination in Arabidopsis. Furthermore, the characterization of the recombinant SFAR4 reveals that it is a novel GDSL-type esterase. These observations suggest that SFAR4 plays an important physiological role in seed germination, seedling growth, and susceptibility to sugar.
Sequence and motif analysis of SFAR4
Using the BLAST program to align the EST database for the SFAR4 (At3g48460) sequence, full-length SFAR4 cDNA was identified. Specific primers were designed to amplify the full-length SFAR4 cDNA. The PCR products were cloned and sequenced. The sequence was subjected to BLAST analysis using the National Center for Biotechnology Information (NCBI) Arabidopsis genomic DNA database to identify the gene structure of SFAR4. The GDSL motif and catalytic triad sites of SFAR4 were characterized according to the SFAR4 sequence alignments to Arabidopsis GLIP1 and the typical GDSL-lipase-like enzymes within the MEGA 4.0 version software. The signal peptide and location of cleavage sites within the amino acid sequences were predicted using the SignalP 4.1 server. Motif analysis was performed with the Multiple Em for Motif Elicitation (MEME) motif search tool.
Construction and transformation of SFAR4 in a yeast expression system
cDNA fragments of SFAR4 were amplified and cloned into a pGAPZαC vector and expressed in Pichia pastoris SMD168H (Invitrogen). P. pastoris was cultured in YPD media (2 % peptone, 1 % yeast extract, 2 % glucose, and 1.5 % agar, pH 7.0). Plasmids were linearized with the restriction enzymes AvrII or BspHI and transformed into P. pastoris SMD168H by electroporation for 5 ms (Chang et al. 2006). High-voltage pulses (1.5 kV) were introduced to 100-µL samples in 0.2-cm electrode gap cuvettes using a BTX Electro Cell Manipulator apparatus. Transformed P. pastoris colonies of constructions were plated on YPD supplemented with zeocin (100 ng L−1, Invitrogen) for colony selection.
Lipase or esterase activity plate assays, purification, and specific activity analysis
Recombinant SFAR4 of P. pastoris transformants were screened using a 1.5 % tributyrin emulsion agar or 2 % Tween-20 plates. The tributyrin solution was sonicated to a milky emulsion before adding the autoclaved YPD media. Tween-20 plates were prepared by using modified YPD media (YPD media supplemented with 0.5 % CaCl2, 2 % Tween-20, pH 7.0) (Cardenas et al. 2001). Lipase activity for tributyrin emulsion was determined by the appearance of clear rings around each colony, whereas for Tween-20, lipase activity was based on the formation of a white calcium precipitate. Colonies showing hydrolytic and lipolytic activity with respect to Tween-20 and tributyrin were isolated and further cultured and purified with HisTrap Ni Sepharose.
Enzyme activity was measured according to Chang et al. (2006). Lipase/esterase activity was assayed by using a spectrophotometer. The hydrolysis of p-nitrophenyl esters was conducted at 30 °C in 500 µL of 50 mM Good’s buffer (50 mM each of bicine, CAPS, sodium acetate, and bis–Tris propane), pH 7.0, containing 0.24 % Triton X-100 and 5 mM p-nitrophenyl esters. The increase in absorbance was recorded for 10 min at a wavelength of 348 nm. One unit of activity was defined as the quantity of enzyme necessary to release 1 µmol of p-nitrophenol per minute. Each experiment was conducted in triplicate. Error bars represented standard deviation (SD).
For antibody preparation, the recombinant SFAR4 protein devoid of the putative signal peptide was amplified and cloned into a pET23a (+) vector and expressed in E. coli BL21 (DE3) competent cells (Novagen). The BL21 (DE3) transformants were cultured in LB media supplemented with ampicillin (50 ng L−1) at 37 °C overnight, then refreshed for 2 h and induced with 0.1 mM of IPTG for 6 h at 37 °C. The cultures were centrifuged to collect bacterial cells, which were then re-suspended in phosphate buffer and sonicated with a Misonix Sonicator XL2020. The sonication condition was 5 s on/off with 50–60 % amplitude on ice for 5 min with a 3-mm probe. Because of inclusion body production, the cell lysate was centrifuged at 20,000×g for 20 min at 4 °C and the pellet was collected. The pellet was washed twice with wash buffer (5 mM imidazole, 0.5 M NaCl, 20 mM phosphate buffer, pH 7.4 and 0.5 % Triton X-100), resuspended in buffer (8 M urea, 0.5 M NaCl, 20 mM phosphate buffer, pH 8.0), and homogenized with a tissue homogenizer. The supernatants following centrifugation (47,800×g, 20 min, 4 °C) were recovered and further purified. The soluble recombinant SFAR4s were purified through a HisTrap affinity column (GE Healthcare Life Sciences). The purified proteins were separated on the SDS–polyacrylamide gel (SDS-PAGE) and stained with Coomassie Brilliant Blue R-250 to confirm the molecular size and the purity. Purified recombinant SFAR4 protein was used for antiserum production. A rabbit polyclonal antibody specific to recombinant SFAR4 was produced by Allbio Science, Inc. (Taichung City, Taiwan), using a standard 70-day rabbit immunization protocol for rabbit polyclonal antibody production.
Plant materials and growth conditions
To study the biological functions of SFAR4 genes, we searched for putative SFAR4 T-DNA insertional mutants from the T-DNA Express database of the SALK Institute Genome Analysis Laboratory (SIGnAL; http://signal.salk.edu/cgi-bin/tdnaexpress) and identified their exact integrated positions. Arabidopsis plants with ecotypes Col-0 were used as wild types for the phenotypic evaluation of plant growth and development. All of the Arabidopsis seeds underwent imbibition for 2 days and were then grown in soil at 22 °C under a 16-h light/8-h dark cycle. For aseptic growth, seeds were surface-sterilized and sown on Murashige and Skoog (MS) medium (Duchefa) and solidified with 0.7 % phytoagar (Duchefa). After sowing, the plates were incubated at 4 °C in the dark for 2 days and subsequently transferred to the growth chamber at 22 °C under 16-h light/8-h dark cycle. For osmotic and salt stress studies, Arabidopsis plants were surface-sterilized and then seeded on MS media plates supplemented with or without salts (NaCl, KCl, or LiCl), mannitol, and glucose at pH 5.7. Growth conditions were as described above. All data are the mean value of at least 50 plants, and these experiments were three replicates, obtaining similar values in each experiment. Data values of cotyledon expansion and greening for seedlings were statistically analyzed using the Student’s t test.
Transgenic plants were generated by Agrobacterium tumefaciens-mediated transformation in Arabidopsis. Full-length SFAR4 was amplified and constructed into pCAMBIA1300-modified vectors that contained the constitutive cauliflower mosaic virus (CaMV) 35S promoter. Then, the constructs were transformed into A. tumefaciens LBA4404 by electroporation. The transformants were screened with the neo gene (kanamycin-resistant) and introduced into plants by floral dip transformation (Clough and Bent 1998). The seeds from the transfected plants were harvested, surface-sterilized, and plated on a hygromycin selection media. Positive seedlings with 4 to 6 adult leaves were then transplanted and maintained in the greenhouse. Homozygous lines were identified in the next generation.
To study the expression pattern of SFAR4, Pro SFAR4 :GUS transgenic plants with the β-glucuronidase (GUS) reporter gene under the control of the SFAR4 promoter were cloned into the pBI101 plasmid, then generated by Agrobacterium tumefaciens-mediated genetic transformation as previously described. The length of SFAR4 promoter is 2.1 kb upstream from the transcription start site. Col-0 wild-type plants were used for the construction of Pro SFAR4 :GUS transgenic plants. An efficient and largely genotype-independent transformation of Arabidopsis was established based on the use of the neo gene as a selectable marker gene. The seeds from the transfected plants were harvested, surface-sterilized, and plated on the kanamycin selection media. Homozygous lines were identified in the next generation and the expression of GUS reporter gene was monitored by the GUS staining method. Staining for GUS activity was performed according to a modified protocol as Schoof et al. (2000) described. Plant material was prefixed in 90 % acetone for 20 min at room temperature, rinsed in staining buffer without 5-bromo-4-chloro-3-indolyl glucuronide (X-Gluc), and infiltrated with staining solution (50 mM NaPO4, pH 7.2; 2 mM potassium-ferrocyanide; 2 mM potassium-ferricyanide; 0.2 % Triton X-100; 2 mM X-Gluc) under vacuum on ice for 15 min and incubated at 37 °C for 3–8 h. After dehydration in an ethanol series up to 70 % EtOH, tissue was stored at room temperature until capturing the images. Photographs were taken with a Zeiss Microscope camera. To analyze the expression pattern of SFAR4, at least 10 independent lines of Pro SFAR4 :GUS T3 homozygous were selected for the GUS staining experiments. In most cases, independent transgenic lines showed similar GUS activity patterns. However, examples of single lines that showed an expression pattern distinct from other lines were also identified. We reasoned that such lines may have the transgene inserted at a genomic site that aberrantly influenced expression or may have experienced a mutation upon transformation. Thus, GUS expression analyses are shown only when at least five independent transgenic lines displayed similar GUS expression patterns.
Isolation of genomic DNA and genomic PCR and Southern blot
Correct T-DNA insertion lines were determined by using genomic PCR. Genomic DNA was isolated from individual plants as described by Winnepenninckx et al. (1995) and amplified by PCR reactions with a set of primers containing SFAR4 gene-specific primers and a T-DNA left border specific primer (Additional file 1: Table S1). PCR products were sequenced and the accurate insertional site of T-DNA was confirmed. These mutants were then determined the T-DNA insertional number by Southern blot.
DNA blot was conducted according to the procedure of Solanas and Escrich (1997) with minor modifications and detected by the DIG-labeling detection system. 10 μg of genomic DNA was digested with a restriction enzyme and separated by 0.8 % agarose gel electrophoresis, and then transferred onto a nylon membrane. The membrane was incubated with a blocking solution for 1 h and then hybridized to a DIG-labeled single-stranded DNA probe overnight at 60 °C. Low stringency washes in 2× SSC/0.1 % SDS for 15 min at 37 °C were performed twice, followed by high stringency washes using 0.2× SSC/0.1 % SDS at 68 °C, following the DIG Application Manual from Roche (South San Francisco, CA, USA).
RT-PCR, and quantitative real-time PCR and Statistical analysis
RNA was isolated from plants by using the RNeasy plant mini kit (Qiagen), and M-MLV reverse transcriptase was employed to generate cDNA. For the reverse transcription reactions, 2 µg of total RNA extracted from 14-day-old seedlings were incubated with 0.5 µg oligo dT in a total of 15 µL at 70 °C for 5 min. After chilling on ice for 5 min, components of the 1× RT buffer (Promega, Madison, WI, USA), 2.5 mM MgCl2, 0.5 mM dNTPs, 25 units of a ribonuclease inhibitor, and 200 units of Superscript RT were added as final concentrations and incubated at 42 °C for 60 min. After incubating at 70 °C for 15 min, four units of RNase H (Promega) were added and the reaction was incubated at 37 °C for 20 min. To examine the expression pattern of SFAR4, 1 μL of cDNA from the RT reactions and the specific primer pair for SFAR4 were used to amplify the full-length cDNA of SFAR4 by PCR.
Quantitative real-time RT-PCR was performed by the CFX Connect™ Real-Time PCR Detection System (Bio-Rad Inc.). Specific primers for each signal are shown in Additional file 1: Table S1. The melting curve was used to confirm the specificity of primers. Dissociation curves of each primer contained a single peak with no shoulders, and the agarose gels of the amplified product revealed single bands corresponding to the predicted amplicon length. All data are the mean value of three independent measures, and the error bars correspond to the standard error. Data values were statistically analyzed using the Student’s t test.
Results and discussion
Identification and characterization of SFAR4
SFAR4, as GDSL-like lipases/esterases, was localized on chromosome 3 and was comprised of 3 exons and 2 introns (Additional file 2: Fig. S1A). The full-length SFAR4 cDNA was 1143 bp in length and encoded a 381-amino-acid protein (approximately 42.3 kDa). The GDSL motif and catalytic triad sites of the SFAR4 protein were characterized by aligning the SFAR4 sequence with the sequences of Arabidopsis GLIP1 and the typical GDSL-lipase-like enzymes using MEGA 4.0 version software. SFAR4 contained conserved motifs of typical GDSL lipase/esterase family proteins, which form the catalytic triad and oxyanion hole (Akoh et al. 2004). Four conserved residues, Ser-Gly-Asn-His, were present in the conserved blocks I, II, III, and V, respectively. Hence, SFAR4 is an SGNH-hydrolase (a subgroup of GDSL esterases/lipases) that contains a conserved GDSL motif near the N-terminus along with the predicted esterase/lipase catalytic triad active sites, Ser45, Asp187, and His347 (Additional file 2: Fig. S1B). The phylogenetic analysis of SFAR4 protein and 24 plant GDSL esterases/lipases (Additional file 3: Fig. S2) indicated that the evolutionary relationship of SFAR4 was homologous to that of BnSCE3/BnLIP2 in Brassica napus, myosuroides hydrolase (AmGDSH1) in the weed black-grass (Alopecurus myosuroides), acetylajmalan esterase (AAE) in Rauvolfia, and ARAB-1 in Arabidopsis (Ling et al. 2006; Cummins and Edwards 2004; Ruppert et al. 2005; Mikleusevic et al. 2009). We suggest that the molecular functions of SFAR4 maybe contain the esterase activity but the physiological function is still unclear.
Purification of enzymes catalyzing the p-nitrophenyl butyrate reaction from Pichia pastoris
Total protein (mg)
Total activity (μM min−1)
Specific activity (μM min−1 mg−1 protein)
Secreted medium (20 times concentrate)
In this study, we found that recombinant SFAR4 protein expressed in E. coli was not active. However, the specific activity analysis of the recombinant SFAR4 protein expressed in yeast showed that it possessed hydrolase activity toward acyl esters (Fig. 1). The recombinant protein expressed in bacteria was deficient in post-translational modifications, whereas that expressed in Pichia pastoris underwent glycosylation modifications. The annotation in the UniPprot database (http://www.uniprot.org/uniprot/Q9STM6) predicted that SFAR4 possessed amino acid modifications in the form of glycosylation at residues 112, 140, and 158. Therefore, we suggested that SFAR4 proteins need post-translational modifications for their activities, such as amino acid glycosylation or correct folding.
Expression pattern of SFAR4
The hormone GA plays a critical role in various processes of plant development, such as seed germination, leaf expansion, stem elongation, flowering, and seed development (Richards et al. 2001; Sun and Gubler 2004). It has been reported that DELLA proteins, which are GA signaling repressors, regulate the expression of numerous GDSL-type lipase/esterase genes during flower and seed development and germination (Cao et al. 2006). It has been demonstrated that five GDSL-type lipase/esterase genes, SFAR1-5, that were down-regulated by DELLA on DELLA mutant transcriptomes, are involved in reducing fatty acid storage and composition in mature Arabidopsis seeds (Chen et al. 2012). However, lipid hydrolysis is not essential for seed germination and seedling establishment (Kelly et al. 2011). Therefore, seed germination and development are distinctly controlled by DELLA-dependent transcriptomes. To analyze the diversity of DELLA-regulated GDSL-type lipase/esterase genes, the (MEME) program was used to elucidate the similarities among the conserved motifs of these proteins. Motifs among DELLA-downregulated GDSL-type enzymes with E-values from 1.02e−1356 to 9.9e−164 were found. Motifs 5, 9, 3, and 1 represent GDSL esterase/lipase conserved blocks I, II, III, and V, respectively. Most DELLA downregulated GDSL-type proteins with conserved motifs 6 and 7 differed significantly from SFAR4 (Additional file 5: Fig. S4). These different domains of SFAR4 may result in distinct activities or functions that are involved in the response of plant physiology to environmental changes.
SFAR overexpression enhanced the germination rate and young seedling establishment during high glucose stress
To study the biological functions of the SFAR4 gene, we searched the Salk Institute database for putative T-DNA insertion mutants of SFAR4 and obtained 2 alleles, sfar4-1 (salk_122440) and sfar4-2 (salk_008418), with T-DNA insertion in exon3 and exon1, respectively. From the SALK database, the T-DNA insertion sites in sfar4-1 and sfar4-2 were deduced to be in exon3 (1341 bp downstream of the ATG start codon) and exon1 (607 bp downstream of the ATG start codon). In this study, the results showed that the exact integrated sites were in exon3 (1567 bp downstream of the ATG start codon) and exon1 (605 bp downstream of the ATG start codon). The insertion site of salk_008418 is consistent with that deduced from the SALK database (Additional file 6: Fig. S5). A Southern blot analysis demonstrated that the sfar4-1 and sfar4-2 lines contained single T-DNA insertions, and RT-PCR revealed that the homozygous mutants of sfar4-1 and sfar4-2 harbored SFAR4 transcripts that underwent gene silencing. To investigate the role of SFAR4 genes in Arabidopsis, full-length cDNAs of transgenic plants expressing SFAR4 under the control of a CaMV 35S promoter were generated through Agrobacterium-mediated transformation. The homozygous overexpressor and knockout mutant lines were confirmed using western blot analysis. The results indicated that the SFAR4 overexpressors exhibited a higher SFAR4 expression level than did the wild-type plants, whereas expression in the knockout mutants (sfar4-1 and sfar4-2) was undetectable. The transgenic plants of two knockout mutants and overexpressors were further used to analyze the variant phenotypes (Additional file 6: Fig. S5).
Expression of HXK1 in SFAR4 transgenic plants during seed germination
SFAR regulates downstream metabolic genes for peroxisomal β-oxidation
In this study, we found that SFAR4 is a GDSL-type esterase that hydrolyzes short-length esters and suggested that SFAR4 requires post-translational modifications for attaining its enzymatic activity. This enzyme played a role in regulating fatty acid degradation through β-oxidation by up-regulating the expression of CTS, LACS6, LACS7, and PNC1 but not PNC2. SFAR4 overexpressors appear to induce tolerance to glucose during germination and seedling growth, and these studies suggest that SFAR4 may play a crucial role in glucose-regulated pathways or glucose metabolism but not HXK1-dependent pathways.
seed fatty acid reducer
L-MH performed all biochemical and molecular biology experiments, and wrote the paper; J-FS and C-PL supervised the research design and wrote the paper; M-TC and L-FOC performed bioinformatics analysis and plant materials. All authors read and approved the final manuscript.
This research was supported by a grant from the National Science Council of Taiwan number NSC 102-2313-B-269-001 to C. P. Lai.
The authors declare that they have no competing interests.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Akoh CC, Lee GC, Liaw YC, Huang TH, Shaw JF (2004) GDSL family of serine esterases/lipases. Prog Lipid Res 43:534–552View ArticlePubMedGoogle Scholar
- Beisson F, Gardies AM, Teissere M, Ferte N, Noat G (1997) An esterase neosynthesized in post-germinated sunflower seeds is related to a new family of lipolytic enzymes. Plant Physiol Biochem 35:761–765Google Scholar
- Bewley JD (1997) Seed germination and dormancy. Plant Cell 9:1055–1066PubMed CentralView ArticlePubMedGoogle Scholar
- Brick DJ, Brumlik MJ, Buckley JT, Cao JX, Davies PC, Misra S, Tranbarger TJ, Upton C (1995) A new family of lipolytic plant enzymes with members in rice, arabidopsis and maize. FEBS Lett 377:475–480View ArticlePubMedGoogle Scholar
- Cao D, Cheng H, Wu W, Soo HM, Peng J (2006) Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in Arabidopsis. Plant Physiol 142:509–525PubMed CentralView ArticlePubMedGoogle Scholar
- Cardenas F, Alvarez E, de Castro-Alvarez MS, Sanchez-Montero JM, Valmaseda M, Elson SW, Sinisterra JV (2001) Screening and catalytic activity in organic synthesis of novel fungal and yeast lipases. J Mol Catal B Enzym 14:111–123View ArticleGoogle Scholar
- Chang SW, Lee GC, Shaw JF (2006) Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase. J Agric Food Chem 54:815–822View ArticlePubMedGoogle Scholar
- Chen MX, Du X, Zhu Y, Wang Z, Hua SJ, Li ZL, Guo WL, Zhang GP, Peng JR, Jiang LX (2012) Seed fatty acid reducer acts downstream of gibberellin signalling pathway to lower seed fatty acid storage in Arabidopsis. Plant Cell Environ 35:2155–2169View ArticlePubMedGoogle Scholar
- Cho YH, Yoo SD, Sheen J (2006) Regulatory functions of nuclear hexokinase1 complex in glucose signaling. Cell 127:579–589View ArticlePubMedGoogle Scholar
- Clauss K, Baumert A, Nimtz M, Milkowski C, Strack D (2008) Role of a GDSL lipase-like protein as sinapine esterase in Brassicaceae. Plant J 53:802–813View ArticlePubMedGoogle Scholar
- Clauss K, von Roepenack-Lahaye E, Bottcher C, Roth MR, Welti R, Erban A, Kopka J, Scheel D, Milkowski C, Strack D (2011) Overexpression of sinapine esterase BnSCE3 in oilseed rape seeds triggers global changes in seed metabolism. Plant Physiol 155:1127–1145PubMed CentralView ArticlePubMedGoogle Scholar
- Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743View ArticlePubMedGoogle Scholar
- Cummins I, Edwards R (2004) Purification and cloning of an esterase from the weed black-grass (Alopecurus myosuroides), which bioactivates aryloxyphenoxypropionate herbicides. Plant J 39:894–904View ArticlePubMedGoogle Scholar
- Dalrymple BP, Cybinski DH, Layton I, McSweeney CS, Xue GP, Swadling YJ, Lowry JB (1997) Three Neocallimastix patriciarum esterases associated with the degradation of complex polysaccharides are members of a new family of hydrolases. Microbiology Uk 143:2605–2614View ArticleGoogle Scholar
- Eastmond PJ, Germain V, Lange PR, Bryce JH, Smith SM, Graham IA (2000) Postgerminative growth and lipid catabolism in oilseeds lacking the glyoxylate cycle. Proc Natl Acad Sci USA 97:5669–5674PubMed CentralView ArticlePubMedGoogle Scholar
- Fait A, Angelovici R, Less H, Ohad I, Urbanczyk-Wochniak E, Fernie AR, Galili G (2006) Arabidopsis seed development and germination is associated with temporally distinct metabolic switches. Plant Physiol 142:839–854PubMed CentralView ArticlePubMedGoogle Scholar
- Footitt S, Slocombe SP, Larner V, Kurup S, Wu YS, Larson T, Graham I, Baker A, Holdsworth M (2002) Control of germination and lipid mobilization by COMATOSE, the Arabidopsis homologue of human ALDP. EMBO J 21:2912–2922PubMed CentralView ArticlePubMedGoogle Scholar
- Footitt S, Marquez J, Schmuths H, Baker A, Theodoulou FL, Holdsworth M (2006) Analysis of the role of COMATOSE and peroxisomal beta-oxidation in the determination of germination potential in Arabidopsis. J Exp Bot 57:2805–2814View ArticlePubMedGoogle Scholar
- Fulda M, Schnurr J, Abbadi A, Heinz E, Browse J (2004) Peroxisomal Acyl-CoA synthetase activity is essential for seedling development in Arabidopsis thaliana. Plant Cell 16:394–405PubMed CentralView ArticlePubMedGoogle Scholar
- Graham IA, Eastmond PJ (2002) Pathways of straight and branched chain fatty acid catabolism in higher plants. Prog Lipid Res 41:156–181View ArticlePubMedGoogle Scholar
- Graham IA, Denby KJ, Leaver CJ (1994) Carbon catabolite repression regulates glyoxylate cycle gene expression in cucumber. Plant Cell 6:761–772PubMed CentralView ArticlePubMedGoogle Scholar
- Hayashi M, Toriyama K, Kondo M, Nishimura M (1998) 2,4-dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects in glyoxysomal fatty acid beta-oxidation. Plant Cell 10:183–195PubMed CentralPubMedGoogle Scholar
- Holdsworth MJ, Finch-Savage WE, Grappin P, Job D (2008) Post-genomics dissection of seed dormancy and germination. Trends Plant Sci 13:7–13View ArticlePubMedGoogle Scholar
- Hong JK, Choi HW, Hwang IS, Kim DS, Kim NH, du Choi S, Kim YJ, Hwang BK (2008) Function of a novel GDSL-type pepper lipase gene, CaGLIP1, in disease susceptibility and abiotic stress tolerance. Planta 227:539–558View ArticlePubMedGoogle Scholar
- Jang JC, Leon P, Zhou L, Sheen J (1997) Hexokinase as a sugar sensor in higher plants. Plant Cell 9:5–19PubMed CentralView ArticlePubMedGoogle Scholar
- Karve A, Xia X, Moore B (2012) Arabidopsis hexokinase-like1 and hexokinase1 form a critical node in mediating plant glucose and ethylene responses. Plant Physiol 158:1965–1975PubMed CentralView ArticlePubMedGoogle Scholar
- Kelly AA, Quettier AL, Shaw E, Eastmond PJ (2011) Seed storage oil mobilization is important but not essential for germination or seedling establishment in Arabidopsis. Plant Physiol 157:866–875PubMed CentralView ArticlePubMedGoogle Scholar
- Koch K (2004) Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr Opin Plant Biol 7:235–246View ArticlePubMedGoogle Scholar
- Li J, Derewenda U, Dauter Z, Smith S, Derewenda ZS (2000) Crystal structure of the Escherichia coli thioesterase II, a homolog of the human Nef binding enzyme. Nat Struct Biol 7:555–559View ArticlePubMedGoogle Scholar
- Ling H, Zhao J, Zuo K, Qiu C, Yao H, Qin J, Sun X, Tang K (2006) Isolation and expression analysis of a GDSL-like lipase gene from Brassica napus L. J Biochem Mol Biol 39:297–303View ArticlePubMedGoogle Scholar
- Linka N, Theodoulou FL, Haslam RP, Linka M, Napier JA, Neuhaus HE, Weber APM (2008) Peroxisomal ATP import is essential for seedling development in Arabidopsis thaliana. Plant Cell 20:3241–3257PubMed CentralView ArticlePubMedGoogle Scholar
- Mikleusevic G, Salopek-Sondi B, Luic M (2009) Arab-1, a GDSL lipase from the model plant, Arabidopsis thaliana (L.) Heynh. Croat Chem Acta 82:439–447Google Scholar
- Molgaard A, Kauppinen S, Larsen S (2000) Rhamnogalacturonan acetylesterase elucidates the structure and function of a new family of hydrolases. Structure 8:373–383View ArticlePubMedGoogle Scholar
- Moore B, Zhou L, Rolland F, Hall Q, Cheng WH, Liu YX, Hwang I, Jones T, Sheen J (2003) Role of the Arabidopsis glucose sensor HXK1 in nutrient, light, and hormonal signaling. Science 300:332–336View ArticlePubMedGoogle Scholar
- Pego JV, Weisbeek PJ, Smeekens SC (1999) Mannose inhibits Arabidopsis germination via a hexokinase-mediated step. Plant Physiol 119:1017–1023PubMed CentralView ArticlePubMedGoogle Scholar
- Penfield S, Rylott EL, Gilday AD, Graham S, Larson TR, Graham IA (2004) Reserve mobilization in the Arabidopsis endosperm fuels hypocotyl elongation in the dark, is independent of abscisic acid, and requires PHOSPHOENOLPYRUVATE CARBOXYKINASE1. Plant Cell 16:2705–2718PubMed CentralView ArticlePubMedGoogle Scholar
- Poirier Y, Ventre G, Caldelari D (1999) Increased flow of fatty acids toward beta-oxidation in developing seeds of Arabidopsis deficient in diacylglycerol acyltransferase activity or synthesizing medium-chain-length fatty acids. Plant Physiol 121:1359–1366PubMed CentralView ArticlePubMedGoogle Scholar
- Richards DE, King KE, Ait-ali T, Harberd NP (2001) How gibberellin regulates plant growth and development: a molecular genetic analysis of gibberellin signaling. Ann Rev Plant Physiol Plant Mol Biol 52:67–88View ArticleGoogle Scholar
- Rolland F, Baena-Gonzalez E, Sheen J (2006) Sugar sensing and signaling in plants: conserved and novel mechanisms. Ann Rev Plant Biol 57:675–709View ArticleGoogle Scholar
- Ruppert M, Woll J, Giritch A, Genady E, Ma X, Stockigt J (2005) Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant–virus expression system. Planta 222:888–898View ArticlePubMedGoogle Scholar
- Schoof H, Lenhard M, Haecker A, Mayer KFX, Jurgens G, Laux T (2000) The stem cell population of Arabidopsis shoot meristems is maintained by a regulatory loop between the CLAVATA and WUSCHEL genes. Cell 100:635–644View ArticlePubMedGoogle Scholar
- Smeekens S (2000) Sugar-induced signal transduction in plants. Ann Rev Plant Physiol Plant Mol Biol 51:49–81View ArticleGoogle Scholar
- Solanas M, Escrich E (1997) An improved protocol to increase sensitivity of Southern blot using dig-labelled DNA probes. J Biochem Biophys Methods 35:153–159View ArticlePubMedGoogle Scholar
- Sun TP, Gubler F (2004) Molecular mechanism of gibberellin signaling in plants. Ann Rev Plant Biol 55:197–223View ArticleGoogle Scholar
- To JP, Reiter WD, Gibson SI (2002) Mobilization of seed storage lipid by Arabidopsis seedlings is retarded in the presence of exogenous sugars. BMC Plant Biol 2:4PubMed CentralView ArticlePubMedGoogle Scholar
- Upton C, Buckley JT (1995) A new family of lipolytic enzymes? Trends Biochem Sci 20:178–179View ArticlePubMedGoogle Scholar
- Winnepenninckx B, Backeljau T, De Wachter R (1995) Phylogeny of protostome worms derived from 18S rRNA sequences. Mol Biol Evol 12:641–649PubMedGoogle Scholar