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Botanical Studies
Fig. 1 | Botanical Studies

Fig. 1

From: An optimum study on the laser scanning confocal microscopy techniques for BiFC assay using plant protoplast

Fig. 1

BiFC signal imaging in protoplast with hardly observed DAPI signal. Gene constructs were co-transfected into Arabidopsis protoplasts to detect their intracellular interaction and localization. A DAPI fluorescence (blue), used as a nuclear marker. B YFP fluorescence (yellow) produced by the BiFC assay. C Bright filed image of the protoplast. Arrow shows the nuclear area. D Autofluorescence (red) of chloroplasts. E Merged image of A, B and D. F Merged image of A–D. All images are shown at the same magnification. Scale bar = 5 µm

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