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Botanical Studies
Fig. 2 | Botanical Studies

Fig. 2

From: An optimum study on the laser scanning confocal microscopy techniques for BiFC assay using plant protoplast

Fig. 2

BiFC signal imaging in protoplast with weak DAPI signal that appeared concurrently with chloroplast signal. A Fluorescence of DAPI and chloroplasts. B YFP fluorescence. C Bright filed image of the protoplast. Arrow shows the nuclear area. D Autofluorescence of chloroplasts. E Merged image of A, B and D. F Merged image of A–D. Dotted circle shows the DAPI signals from the nuclear area, which is inferred from comparison of signals from (A) and (D). All images are shown at the same magnification. Scale bar = 5 µm

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