Fig. 4From: An optimum study on the laser scanning confocal microscopy techniques for BiFC assay using plant protoplastProtoplast with strong signals of both BiFC and DAPI. A DAPI fluorescence. B YFP fluorescence. C Bright filed image of the protoplast. D Autofluorescence of chloroplasts. E Merged image of A, B and D. F Merged image of A–D. All images are shown at the same magnification. Scale bar = 5 µmBack to article page