Fig. 5

Emitting spectra of autofluorescence from Arabidopsis protoplast chloroplasts and excitation spectra of autofluorescence from protoplast suspension. A Chloroplast particles were excited using five laser exciters: 405 nm, 488 nm, 514 nm, 552 nm and 638 nm. The fluorescence signals were detected starting from 420 nm, 500 nm, 530 nm, 570 nm and 650 nm, respectively, and ending at 790 nm. The spectrum width was 25 nm and the step width was 5 nm. Each test was repeated at least six times. B The autofluorescence spectra of Arabidopsis protoplast suspension were monitored using a fluorescence spectrometer with the emission wavelength set at 660 nm