Fig. 7
Light microscopic imaging of protoplasts pipetted with different tips. Protoplasts (25 µL, or a droplet of sample solution) were examined under an inverted microscope with a 20× objective. Images A–C were samples pipetted with original tips of 10 µL, 200 µL and 1 mL, respectively, while images D–F were the samples pipetted with the corresponding tips with ends cut one-third. All images are shown at the same magnification. Scale bar = 50 µm