Figure 2From: Ectopic expression of OsMADS45 activates the upstream genes Hd3a and RFT1 at an early development stage causing early flowering in riceThe diurnal expression patterns of the floral regulatory genes of the Ehd2-OsMADS50-Ehd1-RFT1 and OsGI-Hd1-Hd3a flowering pathways. Leaf RNA samples from 60 DAI of TNG67 rice and Ubi:OsMADS45 transgenic rice (45OX) grown under 14Â h light/10Â h dark cycle condition at 4-hour intervals were collected and analyzed via RT-PCR using gene-specific primers (Additional file 1: Table S1). The actin gene was assessed as an internal control. The black bars indicate the dark period and the white bars indicate the light period.Back to article page