Expression analysis of the floral regulatory genes Ehd2, OsMADS50, Ehd1, RFT1, OsGI, Hd1, Hd3a , OsMADS14 and OsMADS18 in TNG67 and Ubi:OsMADS45 transgenic rice at various growth stages. (A) Representative RT-PCR analysis of the floral regulatory genes expression in TNG67 and Ubi:OsMADS45 transgenic plants (45OX) at various growth stages is shown. Leaf RNA was isolated from the plant samples of TNG67 and Ubi:OsMADS45 transgenic rice (45OX) approximately 2 to 4 hours after or before (for Hd1 gene analysis) dawn at 20, 40, 80 and 120 DAI; the RNA was analyzed via RT-PCR using gene-specific primers (Additional file 1: Table S1). Act1 gene was used as an internal control. (B-K) Semi-quantitative RT-PCR data of OsMADS45 (B), RFT1 (C), Ehd1 (D), OsMADS50 (E), Ehd2 (F), Hd3a (G), Hd1 (H), OsGI (I), OsMADS14 (J), and OsMADS18 (K) in TNG67 (black circle) and 45OX (white circle) plants are shown. The Y axes indicate the relative transcript levels of each gene. The transcript levels were quantified and normalized against actin RNA. The error bars indicate the SE for three replicate experiments.