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Table 1 Effects of PVS2 treatments on the TTC staining and the germination rate of B. formosana cryopreserved seeds

From: Cryopreservation the seeds of a Taiwanese terrestrial orchid, Bletilla formosana (Hayata) Schltr. by vitrification

Seed PVS2 treatment period1
0 min 10 min 20 min 30 min 120 min
TTC staining (%)2 0 85 93 95 94
Germination rate (%)3 0c 78a 69b 72ab 69b
  1. 1Seeds were immersed in loading solution for 30 min and cryopreserved for 30 min.
  2. 2Approximately 300–350 seeds were tested in each TTC staining.
  3. 3Values are means of 5 replicates (200–300 seeds per replicate). Means followed by the same letters are not significantly different by Duncan’s test (P=0.05). Germination rates were calculated after 4 weeks of culture.