Figure 3From: Lemon protein disulfide isomerase: cDNA cloning and biochemical characterizationDouble-reciprocal plot of varying ‘scrambled’ ribonuclease A (sRNase A) on ClPDI activity. The initial rate of the enzymatic reaction was measured with change in absorbance at 260 nm between 1 and 20 min at 20 μg RNA with the sRNase A varied from 3.6 to 21.6 nM. The KM, kcat and kcat/KM were calculated from Lineweaver-Burk plots.Back to article page