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Table 1 Kinetic characterization of ClPDI and that from other sources

From: Lemon protein disulfide isomerase: cDNA cloning and biochemical characterization

Species K M k cat kcat/KM Ref.
(μM) (min-1) (min-1 mM-1)
Lemon 8.3 × 10-3 3.0 × 10-5 3.6 × 10-1 This study
Human* 0.21 ~ 2 0.21 ~ 0.89 1 ~ 0.44 Westphal et al. 1998
Bovine 8.0 ± 1.5 0.46 ± 0.05 5.58 × 10-2 Lyles and Gilbert 1991
Bovine 380 NA NA Lambert and Freedman 1983
Rat 67 ± 7 1.3 ± 0.1 1.94 × 10-2 Walker and Gilbert 1997
  1. Values for PDI are from this work (lemon) or from the literatures (human, bovine, and rat ).
  2. *Human PDI was assayed using fluorescence-quenched peptides as substrate instead of sRNase A.