Molecular analysis of ATM transformed S. miltiorrhiza . (A) PCR analysis of transformerd Salvia plant. Genomic DNA was amplified using hpt II gene specific primers. Approx. 650 bp amplification was observed in transformed plant. Lanes: (M) Molecular weight marker, (1) Positive control (pTAG8 plasmid), (2) Wild type Salvia; and (3) Active mutagenic Salvia plant (SH41). (B) Confirmation of activation-tagged S. miltiorrhiza Bunge by Southern blot analysis. Genomic DNA was extracted from leaves and hpt II gene used as a probe. Hybridization was performed at 52°C. V- pTAG-8; SP- SH41 and WP- WT digested by Pst I; SH- SH41 and WH- WT digested by Hind III; SE- SH41 and WE- WT digested by EcoR I. Arrows indicate hybridization signals.