Activation tagging in Salvia miltiorrhiza can cause increased leaf size and accumulation of tanshinone I and IIA in its roots

Botanical Studies

Table 1 Different solvent extractions of Salvia miltiorrhiza root (commercially available plant)

Compounds	100% CH	70% CH	70% CH/F	100% Et	60% Et	60% Et/F
Danshensu	0.63 ± 0.06	1.05 ± 0.12	1.22 ± 0.02	0.36 ± 0.04	0.98 ± 0.04	1.30 ± 0.04
Protocatechuic	0.10 ± 0.01	0.13 ± 0.01	0.12 ± 0.01	0.09 ± 0.03	0.13 ± 0.13	0.14 ± 0.14
Protocatechuic aldehyde	0.06 ± 0.05	0.06 ± 0.01	0.06 ± 0.03	0.06 ± 0.02	0.06 ± 0.06	0.06 ± 0.06
Caffeic acid	0.06 ± 0.05	0.06 ± 0.02	0.06 ± 0.03	0.05 ± 0.02	0.06 ± 0.06	0.06 ± 0.06
Rosmarinic acid	0.65 ± 0.07	1.08 ± 0.08	1.20 ± 0.02	0.36 ± 0.03	0.96 ± 0.02	1.21 ± 0.04
Salvianolic acid B	3.35 ± 0.09	5.43 ± 0.04	5.97 ± 0.08	1.68 ± 0.08	4.87 ± 0.07	6.08 ± 0.06
Salvianolic acid A	0.25 ± 0.03	0.36 ± 0.04	0.39 ± 0.04	0.16 ± 0.01	0.33 ± 0.03	0.41 ± 0.02
Cryptotanshinone	0.03 ± 0.01	0.06 ± 0.02	0.07 ± 0.02	0.07 ± 0.03	0.06 ± 0.01	0.07 ± 0.02
Tanshinone I	0.08 ± 0.03	0.10 ± 0.04	0.11 ± 0.03	0.18 ± 0.04	0.07 ± 0.02	0.14 ± 0.05
Tanshinone IIA	0.04 ± 0.01	0.07 ± 0.02	0.08 ± 0.02	0.15 ± 0.04	0.07 ± 0.01	0.14 ± 0.03

CH, Methanol; CH/F, Methanol with 0.05% formic acid; Et, Ethanol; Et/F, Ethanol with 0.05% formic acid. Data expressed as mean ± SD for 3 repeats (mg/g).