Characterization of differential expression and leader intron function of Arabidopsis atTOC159 homologous genes by transgenic plants

Botanical Studies

Table 1 Sequences of gene-specific primers used in this study

Gene	Forward primers (5′ to 3′)^a	Reverse primers (5′ to 3′)^a
159PUI	GCCAAGCTT GGATTTGTGTTATGTTTCTCGC	CGGGGATCC CCGCTTTGCTACTGAGACTC
132PUI	GCCAAGCTT TAGCTGCACCAGCTTATTGAG	CGGGGATCC TCTAGATCACCACCGCTACG
132P	GCCAAGCTT TAGCTGCACCAGCTTATTGAG	CGGGGATCC CAGAAGTTAGAGATAGAGAGAG
120PUI	GCCAAGCTT AGTGTGGTGTTGTTTAAGTGTG	CGGGGATCC CTAGGATCACCCAAAATCACG
120P	GCCAAGCTT AGTGTGGTGTTGTTTAAGTGTG	CGGGGATCC CTGGTTAGAGAAGGCAAAAGTC
90PUI	GCCAAGCTT AGACGAAGATGTCGTCATTGG	CGGGGATCC AACTATCTGCCCAACAGCAAG
90P	GCCAAGCTT AGACGAAGATGTCGTCATTGG	CGGGGATCC TTGTGTTGGCGAGAGAAAGAG

^aThe underlined sequences are additional restriction sites. Bam HI, GGATCC; Hind III, AAGCTT.