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Table 1 Effect of cytokinins and Aloe vera leaf gel (AvG) supplemented with MS basal medium on shoots regeneration of Aloe vera

From: High frequency microcloning of Aloe vera and their true-to-type conformity by molecular cytogenetic assessment of two years old field growing regenerated plants

MS medium + Supplement

1stregeneration cycle

3rdregeneration cycle

Supplement types

Supplement Conc.

Response (%)

Number of shoot (≥ 2.0 cm) per explant

Response (%)

Number of shoot (≥ 2.0 cm) per explant

Control

Without PGRs/AvG

0

0

0.0 ± 0.0a

93.3

1.0 ± 0.0a

Cytokinin (mg/L)

BAP

1.0

73.3

8.3 ± 0.23c

100

16.2 ± 0.34c

2.5

80.0

14.5 ± 0.31f

100

27.6 ± 0.53h

4.0

66.7

11.7 ± 0.44e

100

23.1 ± 0.44f

KIN

1.0

63.3

5.2 ± 0.24b

100

12.8 ± 0.28b

2.5

76.7

8.2 ± 0.24c

100

17.7 ± 0.40d

4.0

70.0

9.7 ± 0.29d

100

20.3 ± 0.33e

Cytokinin (mg/L) + AvG (%)

BAP + AvG

2.5 + 5

93.3

15.9 ± 0.24g

100

33.3 ± 0.41j

2.5 + 10

96.7

17.8 ± 0.35h

100

38.5 ± 0.44k

2.5 + 15

93.3

14.3 ± 0.28f

100

30.9 ± 0.43i

2.5 + 20

90.0

12.5 ± 0.30e

100

24.3 ± 0.41g

  1. Each value represents the means ± SE, n = 30. Means followed by the same letters in each column are not significantly different at P < 0.05 according to Duncan’s multiple range tests.