MS medium + Supplement | 1stregeneration cycle | 3rdregeneration cycle | |||
---|---|---|---|---|---|
Supplement types | Supplement Conc. | Response (%) | Number of shoot (≥ 2.0 cm) per explant | Response (%) | Number of shoot (≥ 2.0 cm) per explant |
Control | |||||
Without PGRs/AvG | 0 | 0 | 0.0 ± 0.0a | 93.3 | 1.0 ± 0.0a |
Cytokinin (mg/L) | |||||
BAP | 1.0 | 73.3 | 8.3 ± 0.23c | 100 | 16.2 ± 0.34c |
2.5 | 80.0 | 14.5 ± 0.31f | 100 | 27.6 ± 0.53h | |
4.0 | 66.7 | 11.7 ± 0.44e | 100 | 23.1 ± 0.44f | |
KIN | 1.0 | 63.3 | 5.2 ± 0.24b | 100 | 12.8 ± 0.28b |
2.5 | 76.7 | 8.2 ± 0.24c | 100 | 17.7 ± 0.40d | |
4.0 | 70.0 | 9.7 ± 0.29d | 100 | 20.3 ± 0.33e | |
Cytokinin (mg/L) + AvG (%) | |||||
BAP + AvG | 2.5 + 5 | 93.3 | 15.9 ± 0.24g | 100 | 33.3 ± 0.41j |
2.5 + 10 | 96.7 | 17.8 ± 0.35h | 100 | 38.5 ± 0.44k | |
2.5 + 15 | 93.3 | 14.3 ± 0.28f | 100 | 30.9 ± 0.43i | |
2.5 + 20 | 90.0 | 12.5 ± 0.30e | 100 | 24.3 ± 0.41g |