High frequency microcloning of Aloe vera and their true-to-type conformity by molecular cytogenetic assessment of two years old field growing regenerated plants

Botanical Studies

Table 2 Effect of the strength of MS medium and concentration of sucrose (S) and Aloe vera leaf gel (AvG) on in vitro rooting of Aloe vera (after 18 d of implantation)

Strength of MS medium, concentration of sucrose (w/v) and AvG (v/v)	Percentage of shoot showing root formation	Number of root per shoot [means ± SE]	Length of longest root per shoot (cm) [means ± SE]
Full MS + 3% S	56.7	3.3 ± 0.25^a	1.5 ± 0.12^a
Two third MS + 2% S	70.0	4.2 ± 0.23^b	1.9 ± 0.08^b
One third MS + 1% S	76.7	5.6 ± 0.26^c	2.3 ± 0.08^c
Water-agar medium*	20.0	2.7 ± 0.33^a	2.9 ± 0.14^d
One third MS + 1% S + 10% AvG	86.7	7.3 ± 0.24^d	2.4 ± 0.07^c
One third MS + 1% S + 20% AvG	100	9.8 ± 0.29^e	3.1 ± 0.10^d
One third MS + 1% S + 30% AvG	100	9.2 ± 0.26^e	2.8 ± 0.08^d
One third MS + 1% S + 40% AvG	93.3	6.5 ± 0.23^d	2.1 ± 0.10^bc

Each value represents the means ± SE, n = 30. Means followed by the same letters in each column are not significantly different at P < 0.05 according to Duncan’s multiple range tests.
*Medium without MS nutrient and sucrose but solidified with agar.