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Table 1 Effect of plant growth regulators (PGRs) on callus induction from mature seed after 28 days of incubation

From: Rapid regeneration and ploidy stability of ‘cv IR36’ indica rice (Oryza Sativa. L) confers efficient protocol for in vitro callus organogenesis and Agrobacterium tumefaciens mediated transformation

Concentration of 2,4-D (μM)

Concentration of Kn (μM)

Concentration of BAP (μM)

Average callus induction (%)

Average mean F.Wt (mg)

Control (MS alone)

-

-

6.25 ± 0

41.3 ± 2.1

11.31

-

-

93.75 ± 6.25*

140.24 ± 0.9*

11.31

0.02

-

63.17 ± 0.76

154.73 ± 2.44

11.31

0.20

-

87.00 ± 0.50

148.70 ± 5.28

11.31

0.30

-

98.00 ± 0.87*

161.28 ± 1.28*

11.31

0.40

-

62.00 ± 0.50

157.03 ± 4.87

11.31

0.50

-

55.42 ± 0.88

150.05 ± 5.93

11.31

0.60

-

44.58 ± 0.88*

151.29 ± 8.52

11.31

0.70

-

36.67 ± 0.76*

146.31 ± 3.61

11.31

0.80

-

98.17 ± 0.29

146.56 ± 3.78

11.31

0.90

-

43.08 ± 0.95*

150.46 ± 7.37

11.31

0.22

-

11.67 ± 0.76*

142.83 ± 1.06

11.31

-

0.44

85.00 ± 3.50

154.09 ± 4.67

11.31

-

0.88

73.16 ± 2.02

147.13 ± 6.37

11.31

-

1.32

76.08 ± 2.79

148.87 ± 0.89*

11.31

-

1.76

52.16 ± 2.02*

135.43 ± 10.79

11.31

-

2.20

50.66 ± 3.06*

146.52 ± 8.93

11.31

-

2.64

58.83 ± 3.33*

144.79 ± 4.21

11.31

-

3.08

79.66 ± 1.53

150.50 ± 8.08

11.31

-

3.52

75.83 ± 1.04

140.67 ± 0.50

11.31

-

3.96

68.75 ± 0.00

142.65 ± 12.23

11.31

-

4.40

61.5 ± 1.32

128.50 ± 0.47*

  1. -Nil.
  2. Values represent means ± standard deviation; * indicates significance at the level of P < 0.5.