Rapid regeneration and ploidy stability of ‘cv IR36’ indica rice (Oryza Sativa. L) confers efficient protocol for in vitro callus organogenesis and Agrobacterium tumefaciens mediated transformation

Botanical Studies

Table 4 Nuclear DNA content assessment between wild type and tissue culture derived IR36 samples

Material donor*	Number of days old	DNA fluorescence index¹	Average mean ploidy²	Average nuclear DNA content³(pg/2c)	Average 2c genome size⁴ (Mbps)
Germinated	40	0.942	25798.33	0.85	829.38
In vitro Germinated	45	0.881	24114.00	0.79	775.23
In vitro Regenerated	45	0.873	23901.00	0.79	768.39
Hardened	40	0.887	24277.00	0.80	780.47

^*Sample Size: Three samples each.
¹DNA fluorescence index (DI = 2CIR36 sample/2CIR36 wild type) (Alan et al. 2007).
²Average PI-A mean value of P2 population.
^3. $SamplePloidy (integer) = ReferencePloidy \times \frac{MeanpositionoftheG 1 samplepeak}{MeanpositionoftheG 1 referencepeak}$
⁴DNA content 1 pg DNA = 978 Mbp (Dolezel et al. 2003.