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Table 4 Nuclear DNA content assessment between wild type and tissue culture derived IR36 samples

From: Rapid regeneration and ploidy stability of ‘cv IR36’ indica rice (Oryza Sativa. L) confers efficient protocol for in vitro callus organogenesis and Agrobacterium tumefaciens mediated transformation

Material donor* Number of days old DNA fluorescence index1 Average mean ploidy2 Average nuclear DNA content3(pg/2c) Average 2c genome size4 (Mbps)
Germinated 40 0.942 25798.33 0.85 829.38
In vitro Germinated 45 0.881 24114.00 0.79 775.23
In vitro Regenerated 45 0.873 23901.00 0.79 768.39
Hardened 40 0.887 24277.00 0.80 780.47
  1. *Sample Size: Three samples each.
  2. 1DNA fluorescence index (DI = 2CIR36 sample/2CIR36 wild type) (Alan et al. 2007).
  3. 2Average PI-A mean value of P2 population.
  4. 3. SamplePloidy integer = ReferencePloidy × MeanpositionoftheG 1 samplepeak MeanpositionoftheG 1 referencepeak
  5. 4DNA content 1 pg DNA = 978 Mbp (Dolezel et al. 2003.