Figure 5
Cytotoxicity and neurocytoprotective activity of the isolated constituents of H. pogonocalyx in 6-OHDA-induced NGF-differentiated PC12 cells. (A) NGF-differentiated PC12 cells (2 × 105) were treated with 6-OHDA (175 μM) and tested samples (100 μM) for 24 h. (B) The NGF-differentiated PC12 cells (2 × 105) were treated with the tested constituents (100 μM) for 6 h and then were treated with 6-OHDA (175 μM) for 24 h. The cell-protective activity was calculated as follows: (OD450 of the sample/OD450 of 6-OHDA) × 100. Each determination was made in triplicate, and the data shown represent means ± SD. *P-value < 0.05 when compared with control.