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Table 1 454 Pyrosequencing results for the pepper ( Capsicum annuum ) varieties, Saengryeg 211 and Saengryeg 213

From: De novo transcriptome assembly and novel microsatellite marker information in Capsicum annuum varieties Saengryeg 211 and Saengryeg 213

454 pyrosequencing terms

Saengryeg 211

Saengryeg 213

Reads

  

 The Number of raw sequencing reads (n)

361,671

274,269

 Bases of raw sequencing reads (bp)

164,494,414

124,608,071

 The average of read length (bp)

454,818

454,328

 Assembleda

282,705

217,905

 Partialb

50,330

33,427

 Singletonsc

18,147

15,129

 Repeatd

111

242

 Outliere

5,075

3,699

 Too shortf

5,273

3,831

 The number of bases, Q20 ≤ (bp)g

93,272,495

70,812,087

 The number of reads, Q20 ≤ (n)

269,198

205,166

Isotigs

  

 Number of isotigs (n)

23,821

17,813

 Average count of contigs in the isotigs (n)

1.401

1.317

 Largest count of contigs in the isotigs (n)

12

16

 The number of isotigs assembled by one contig (n)

18,363

14,556

 The total number of bases in the isotig (bp)

20,787,054

14,965,217

 The average isotig size (n)

872.636

840.129

 N50 isotig size (bp)h

1,028

977

 The size of the largest isotig (bp)

17,462

7,300

Singletons

  

 Number of singletons after SeqClean (minimum length, 100)

18,147

15,129

 Number of singletons after Lucy (minimum length, 100)

17,054

14,310

 Number of valid singletons after Lucy

16,958

14,244

  1. aThe number of read’s bases used in the assembly computation.
  2. bOnly part of the read was included in the assembly.
  3. cThe read did not overlap with any other reads in the input.
  4. dThe read deemed to be from repeat regions.
  5. eThe read was identified by the GS De Novo Assembler as problematic.
  6. fThe read was too short to be used in the computation.
  7. gThe quality of read was higher than or equal to 20.
  8. hHalf of all bases reside in contigs of this size or longer.