Figure 4

Effects of acteoside (A), isoacteoside (I), caffeic acid (C), and 3′,4′-dihydroxyphenylethanol (D) on anti-argpyrimidine (anti-AP) formatiopns in different incubation days (2 or 3 days) at BSA/methylglyoxal (MGO) models. (A) The AP formations in BSA under different MGO concentrations (1 or 5 mM in the final concentrations) for 2 or 3 days using ELISA methods detected by anti-argpyrimidine (anti-AP) antibody; (B) Effects of 0.5 or 5 mM concentrations of A, I, C, and D on anti-AP formations for 3-day in BSA/MGO (5 mM) models detected by ELISA methods; (C) The immune stains on anti-AP formations for 3-day in BSA/MGO (5 mM) models detected by anti-AP antibody. The mean ± SD values were calculated from triplicate measurements. The difference between the control and the experimental group at the same concentration was analyzed using Student’s t-test, and the P-value of less than 0.05 (*), 0.01 (**), and 0.001 (***) were recognized as different significantly.