Marker* | Forward primer | Reverse primer | Size (bp) | Annealing temp. | Nuclease | Size of major bands (bp) | Allele number | PIC§ | Reference sequence※ | Prediction function |
---|---|---|---|---|---|---|---|---|---|---|
C01 | GAGGGGAAGGATGGATTGTT | GTGCCACAAATGACCTACGA | 677 | 55°C | Taq I | A: 677 B: 552 + 125 | 2 | 0.15 | AY741470 | photosystem II CP43 protein |
C02 | GAGGGGAAGGATGGATTGTT | GTGCCACAAATGACCTACGA | 677 | 55°C | Bsr DI | A: 677 B: 483 + 194 | 2 | 0.13 | AY741470 | photosystem II CP43 protein |
C03 | GAGAGAGAGGGATTCGAACC | GTTTTTGGAGCTGGGATGAA | 659 | 55°C | Sfa NI | A: 659 B: 449 + 210 | 2 | 0.27 | AY839880 | trnS ~ trnfM |
M01 | TGGTGAGGAGCATTGTTTTG | GAGCAAACACTCGAACGTGA | 836 | 55°C | Eco RI | A: 836 B: 509 + 327 | 2 | 0.15 | AY839898 | NADH dehydrogenase subunit 1 |
M02 | CCAATTTTTGGGCCAATTCC | TCTCTAAAGGGGCGTAAGCA | 610 | 55°C | Hinc II | A: 610 B:385 + 225 | 2 | 0.31 | AY845285 | NADH dehydrogenase subunit 5 |
M03 | GCCGGAAAAATAACAGACGA | AAAAGGAAGGTTGGGTGCTT | 458 | 55°C | Bbs I | A: 458 B: 308 + 149 | 2 | 0.28 | AY845314 | NADH dehydrogenase subunit 7 |
M04 | GATAGGAGCATTCGGTGGAA | CGGTAACCAAAGCGTATCGT | 844 | 55°C | Hph I | A: 805 + 35 B: 492 + 313 + 35 | 2 | 0.28 | AY845314 | NADH dehydrogenase subunit 7 |
M05 | ACAGCACCTTTTTCCCCTCT | CATAACACGGCTCTCCCACT | 686 | 55°C | Xmn I | A: 686 B:443 + 243 | 2 | 0.34 | AY845314 | NADH dehydrogenase subunit 7 |
M06 | TGAATGAATCCCATCCCCTA | GGCATACAACCGAAACGACT | 413 | 55°C | Rsa I | A: 413 B:309 + 104 | 2 | 0.35 | AY845285 | NADH dehydrogenase subunit 5 |
M07 | TAGCTATGCCCTGCTTGGTC | CCTGTCTGTCGTACCGTTGA | 667 | 55°C | Bss SI | A: 667 B: 460 + 207 | 2 | 0.28 | AY845298 | NADH dehydrogenase subunit 7 |
G01 | TGCTTTGCGTCAATAACTGC | TGATACATCCTCGCCAACAA | 647 | 55°C | Hph I | A:647 B:441 + 206 | 2 | 0.25 | DQ869863 | zinc finger protein |
G02 | GCCTATCTAATCTACTCGGCTTTCT | AGTAACACTAACCCACCCAACAATA | 834 | 55°C | Fsp I | A:834 B:645 + 189 | 2 | 0.36 | AB117640 | ammonium transporter |
G03 | GCCTATCTAATCTACTCGGCTTTCT | AGTAACACTAACCCACCCAACAATA | 834 | 55°C | Ava I | A:834 B:729 + 105 C:501 + 228 + 105 | 3 | 0.55 | AB117640 | ammonium transporter |
G04 | GAGACAGAGGACTACTTCGATTCAG | GAATCAGAAATGATACAGAGGAGGA | 721 | 55°C | Mse I | A:721 B:447 + 274 | 2 | 0.37 | AB247282 | cyclin D3-1 |
G05 | GAGACAGAGGACTACTTCGATTCAG | GAATCAGAAATGATACAGAGGAGGA | 721 | 55°C | Rsa I | A:721 B:571 + 150 | 2 | 0.30 | AB247282 | cyclin D3-1 |
G06 | GCCTATCTAATCTACTCGGCTTTCT | AGTAACACTAACCCACCCAACAATA | 834 | 55°C | Nde I | A:834 B:749 + 85 | 2 | 0.25 | AB117640 | ammonium transporter |
G07 | GCCTATCTAATCTACTCGGCTTTCT | AGTAACACTAACCCACCCAACAATA | 834 | 55°C | Hph I | A:510 + 147 B:468 + 147 + 120 + 57 | 2 | 0.31 | AB117640 | ammonium transporter |
G08 | GCCTATCTAATCTACTCGGCTTTCT | AGTAACACTAACCCACCCAACAATA | 834 | 55°C | Ava II | A:443 + 190 + 134 + 67 B: 324 + 67 | 2 | 0.15 | AB117640 | ammonium transporter |
G09 | GCCTATCTAATCTACTCGGCTTTCT | AGTAACACTAACCCACCCAACAATA | 834 | 55°C | Bst UI | A:575 + 204 + 55 B:455 + 204 + 120 + 55 | 2 | 0.07 | AB117640 | ammonium transporter |
G10 | TGAGACAACATTATGGTCGATAGAA | ATACTCCTTGCAAACTTCTGAATTG | 750 | 55°C | Bst YI | A:750 B:590 + 160 | 2 | 0.14 | AY641731 | trans-cinnamate 4-hydroxylase |
G11 | TGAGACAACATTATGGTCGATAGAA | ATACTCCTTGCAAACTTCTGAATTG | 750 | 55°C | Bst UI | A:750 B:470 + 280 | 2 | 0.10 | AY641731 | trans-cinnamate 4-hydroxylase |
G12 | ACGACTACAGCTTCTTTCTCTACCA | ATACACCTCGTCGACATACTTCTTC | 824 | 55°C | Ava I | A:824 B: 527 + 297 C:325 + 202 | 3 | 0.43 | AB114913 | ammonium transporter |
G13 | CCATCAAATCCATTGGGAAC | AAGACGAGCCAGGAGAAACA | 580 | 60°C | Mbo II | A:580 B:449 + 131 | 2 | 0.10 | EF205149 | 1-aminocyclopropane-1-carboxylate synthase |
G14 | CCATCAAATCCATTGGGAAC | AAGACGAGCCAGGAGAAACA | 580 | 60°C | Bsr DI | A:359 + 221 B:455 + 125 C:221 + 218 + 141 D:260 + 195 + 125 E:359 + 125 + 96 F:455 + 320 + 260 + 125 G:260 + 125 + 99 + 96 | 7 | 0.52 | EF205149 | 1-aminocyclopropane-1-carboxylate synthase |
G15 | GCCTATCTAATCTACTCGGCTTTCT | AGTAACACTAACCCACCCAACAATA | 834 | 55°C | Bsa JI | A:546 + 288 B:318 + 288 + 228 C:546 + 183 + 105 D: 318 + 228 + 183 + 105 | 5 | 0.58 | AB117640 | ammonium transporter |
G16 | CCTACAAAACAGTCATAAGCCAACT | ACGAAAACACTCTTGATCAGTAAGG | 572 | 55°C | Bbv I | A:309 + 263 B:263 + 166 + 143 | 2 | 0.04 | AB015047 | PR-1 like protein |
G17 | ACGTGTGTGTTTCATTTGCC | AACCCAATGATGTGTAAGTG | 556/401 | 55°C | Mbo II | A:556 B:455 + 101 C:300 + 101 | 3 | 0.28 | D26596b | phenylalanine ammonia-lyase |
G18 | CTTACGGCTCTCGCAGAAGA | GAACCGTGATCCAGGTTTTG | 1100/930 | 55°C | Hind III | A:1100 B:930 C:650 + 280 | 3 | 0.49 | AY641730 | flavanone 3-hydroxylase |
G19 | TGCTTTGCGTCAATAACTGC | TGATACATCCTCGCCAACAA | 647 | 55°C | Hpa II | A:619 + 28 B:463 + 156 + 28 | 2 | 0.37 | DQ869863 | zinc finger protein |
G20 | ACGTGTGTGTTTCATTTGCC | AACCCAATGATGTGTAAGTG | 556/401 | 55°C | Hph I | A:556 + 401 B:401 C:319 + 237 D:237 | 4 | 0.47 | D26596a | phenylalanine ammonia-lyase |
G21 | ACGTGTGTGTTTCATTTGCC | AACCCAATGATGTGTAAGTG | 556/401 | 55°C | Taq I | A:556 B:460 + 96 C:305 + 96 | 3 | 0.53 | D26596a | phenylalanine ammonia-lyase |
G22 | CTTACGGCTCTCGCAGAAGA | GAACCGTGATCCAGGTTTTG | 1100/930 | 55°C | Bst YI | A:930 B:680 + 250 C:510 + 420 D:680 + 420 | 4 | 0.62 | AY641730 | flavanone 3-hydroxylase |
G23 | CTTACGGCTCTCGCAGAAGA | GAACCGTGATCCAGGTTTTG | 1100/930 | 55°C | Bbs I | A:1100 B:930 C:600 + 330 | 3 | 0.55 | AY641730 | flavanone 3-hydroxylase |
G24 | CCAGGAACACCAACAACCCGT | CCATGCTGCTTTCTCTGCCAA | 958 | 55°C | Hind III | A:958 B:550 + 408 | 2 | 0.35 | AB018685b | dihydroflavonol 4-reductase |
G25 | GATCCTTCAGACATGCAGAGC | CACTTCCTCAAGTGATGCAAA | 960 | 55°C | Rsa I | A:960 B:605 + 355 | 2 | 0.21 | EF526217 | caffeine synthase |
G26 | CGACCATCCTGCAATTTTCT | AACGTCATTAGGACCTTCAATCG | 299 | 55°C | Taq I | A:310 B:299 C:290 D:277 | 4 | 0.49 | EF205148 | leucoanthocyanidin reductase |
G27 | GGTGCTCAGGACATGGTTTT | CGCTCTATTCCCTGCAAGTC | 377 | 55°C | Hph I | A:377 B:198 + 179 | 2 | 0.37 | DQ194358 | flavonoid 3′,5′-hydroxylase |
PAL | ACGTGTGTGTTTCATTTGCC | AACCCAATGATGTGTAAGTG | 556/401 | 55°C | - | A:556 B:401 | 2 | 0.27 | D26596a | phenylalanine ammonia-lyase |
F3H | CTTACGGCTCTCGCAGAAGA | GAACCGTGATCCAGGTTTTG | 1100/930 | 55°C | - | A:1100 B:930 | 2 | 0.28 | AY641730 | flavanone 3-hydroxylase |