Skip to main content
Botanical Studies

Table 1 Effect of different PGRs on hairy root production and secondary metabolite accumulation in Gentiana scabra in liquid B5 medium*

From: Establishment of hairy root lines and analysis of iridoids and secoiridoids in the medicinal plant Gentiana scabra

PGR constituents (1 mg/L)

Average dry weight after 4 weeks (mg)**

Weight gain (folds)

Loganic acid (mg/g of dw)**

Sertiamarin (mg/g of dw)**

Gentiopicroside (mg/g of dw)**

0

188.67 ± 8.08 a

35.60

0.78 ± 0.11 c

1.70 ± 0.24 c

43.26 ± 2.28 b

IBA

148.00 ± 9.54 b

27.92

2.86 ± 0.09 b

1.36 ± 0.12 d

32.66 ± 1.71 c

NAA

154.00 ± 20.78 b

29.06

1.53 ± 0.17 d

2.68 ± 0.06 a

55.19 ± 0.74 a

TDZ

115.67 ± 5.86 c

21.82

ND

0.35 ± 0.01 e

10.03 ± 0.13 d

Zeatin

156.50 ± 16.26 b

29.53

4.20 ± 0.02 a

2.43 ± 0.07 b

51.88 ± 0.89 a

  1. *B5 basal salts supplemented with 3% sucrose. Observations were recorded after 4 weeks of culture.
  2. **Values are the means ± standard deviation (n = 3). Means within column followed by the same letter are not significantly different at the 5% probability level (p ≤ 0.05) by LSD (least significant difference) test.
  3. dw: Dry weight.
  4. ND: Non detectable.
Back to article page