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Table 1 Effect of different PGRs on hairy root production and secondary metabolite accumulation in Gentiana scabra in liquid B5 medium*

From: Establishment of hairy root lines and analysis of iridoids and secoiridoids in the medicinal plant Gentiana scabra

PGR constituents (1 mg/L) Average dry weight after 4 weeks (mg)** Weight gain (folds) Loganic acid (mg/g of dw)** Sertiamarin (mg/g of dw)** Gentiopicroside (mg/g of dw)**
0 188.67 ± 8.08 a 35.60 0.78 ± 0.11 c 1.70 ± 0.24 c 43.26 ± 2.28 b
IBA 148.00 ± 9.54 b 27.92 2.86 ± 0.09 b 1.36 ± 0.12 d 32.66 ± 1.71 c
NAA 154.00 ± 20.78 b 29.06 1.53 ± 0.17 d 2.68 ± 0.06 a 55.19 ± 0.74 a
TDZ 115.67 ± 5.86 c 21.82 ND 0.35 ± 0.01 e 10.03 ± 0.13 d
Zeatin 156.50 ± 16.26 b 29.53 4.20 ± 0.02 a 2.43 ± 0.07 b 51.88 ± 0.89 a
  1. *B5 basal salts supplemented with 3% sucrose. Observations were recorded after 4 weeks of culture.
  2. **Values are the means ± standard deviation (n = 3). Means within column followed by the same letter are not significantly different at the 5% probability level (p ≤ 0.05) by LSD (least significant difference) test.
  3. dw: Dry weight.
  4. ND: Non detectable.