Table 4 Retention time, maximum UV absorption, and molecular weight, flavonoid contents (μg/g DW) in the di-, mix- and tetraploid plants and identification methods of phenolic and flavonoid compounds of D. kotschyi
Peak | Compound name | RT (min) | UV (nm) | (m/z)[M-H]-/[M-H]+ | Diploid | Mixploid | Tetraploid | Identification methods |
|---|---|---|---|---|---|---|---|---|
1 | Luteolin-7-O-β-D- glucopyranoside | 1.66 | 205, 255–266, 348 | 447.09/ - | 64.92 | 116.63 | 93.82 | UV, MS, Ref. |
2 | Apigenin 7-O-glucoside (cosmosiin) | 9.20 | 233, 269 | 431.10/ - | 32.72 | 28.59 | 26.63 | UV, MS, Ref. |
3 | Rosmarinic acid | 16.65 | 234, 290, 329 | 359.07/ - | 938.82 | 963.96 | 952.33 | UV, MS, Ref., St. |
4 | Luteolin 3′-O-β-D-glucuronide | 17.11 | 236, 267, 340 | 461.07/ - | 246.48 | 246.27 | 252.05 | UV, MS, Ref. |
5 | Luteolin | 20.13 | 232, 267, 344 | 285.04/ - | 20.96 | 15.78 | 24.97 | UV, MS, Ref., St. |
6 | Apigenin | 22.46 | 232,268,337 | 269.04/ - | 43.15 | 42.26 | 35.86 | UV, MS, Ref., St. |
7 | Cirsimaritin | 23.2 | 276, 232,234 | 313.07/ - | 45.87 | 42.38 | 36.48 | UV, MS, Ref., St. |
8 | Isokaempferide | 24.68 | 232, 266, 350 | 299.05/ - | 54.86 | 31.57 | 54.35 | UV, MS, Ref., St. |
9 | Penduletin | 26.36 | 234, 272, 340 | 343.08/345.09 | 44.69 | 16.33 | 80.21 | UV, MS, Ref. |
10 | Xanthomicrol | 27.7 | 232, 282, 333 | 343.08/345.09 | 81.75 | 31.09 | 140.17 | UV, MS, Ref., St. |
11 | Calycopterin | 30.24 | 233, 278, 337 | 373.09/375.10 | 9.06 | 61.41 | 193.20 | UV, MS, Ref., St. |