Figure 5

PCR-dependent genotyping of homogeneity of the inserted COKC in 6 rice plants regenerated from SA-induced anther culture from their starter lines K19, K17, K20 and K24. (a) Site-specific primers (solid triangle) were designed according the flanking sequences of each COKC insertion locus. These primers were combined with COKC specific primers (gray triangle) for multiplex PCR yielding a unique product of sizes of 500 or 350 bp for corresponding COKC-containing (b) or empty (c) sites, respectively. Both products were amplified only when mixing DNAs of each starter line and its regenerated progeny by anther culture (d).