Figure 4

Transcriptional analysis of d4h-like gene. a) Expression profile of d4h-like in C. roseus plant and suspension cells. RSP9 was used as the reference control. b) MeJA effect on the d4h-like transcription. C₂₀hi cells cultured for 14 d just before adding MeJA were used as control. c) Semi-quantitative RT-PCR result of d4h-like by addition of 2,4-D into the media. 1: C₂₀hi cells in B5 basic media; 2:C₂₀hi cells in B5 media adding of 2,4-D; “32×” and “28×” meant the PCR amplification cycle was 32 and 28, respectively. d) GUS transient expression assay. Left panel represented CaMV35S and 5′-deletion d4h-like promoter fragments constructions. Right panel displayed the corresponding GUS activities. Double stars indicated the difference between the two samples was significant. Each sample was triplicates.