Time-course changes in xenobiotic metabolizing enzyme activities and GSH level in rats treated with APAP in compare to negative controls. A) GST activities B) CYP450 activities C) GSH levels. In negative control group (NC), the APAP vehicle i.e. 400 μl DMSO was only injected. In control group (C), the acetaminophen (500 mg/kg bw) dissolved in 400 μl DMSO was i.p injected. Data are mean ± S.E.M. of five samples obtained from five animals in each group. (*) denote significantly different from the respective negative control group (P < 0.05).