Figure 7
Effect of A. wilhelmsii essential oils on histopathological changes 24 h after APAP administration. Light microscopy showed histologic sections of liver of rats from different groups of animals. A: Section of liver from negative control group (NC). Normal structure of liver tissue with normal central vein (stars) is detected. Normal sinusoid (arrow) between hepatocyts (arrow head) which form hepatic cords (A 1 : original magnification ×40, A 2 : ×100 and A 3 : ×400). B: Section of liver from control group (C) (acetaminophen (500 mg/kg bw)). B1: Liver section with necrotic hyalinized aggregation in perilobular zone (arrow) and disarray of hepatic lobule and hepatic cords (B 1 : original magnification × 100). B 2 : This section shows hepatocytes necrosis with acidophil bodies (white arrow) and kariolysis (black arrow) in necrosis area. Their nucleuses are comparative with around normal hepatocyte nucleus. The hepatocyte cytoplasm, hepatic sinusoid and hepatic cord are disarray (B 2 : original magnification ×400). C: Section of livers from treatment group (APAP+ 100 mg/kg b.w essential oils) C 1 : The necrotic regions were not observed and sinusoids (arrows), hepoatocytes and veins (star) are relatively normal (original magnification ×40). C 2 : Section from preportal and portal space which triad [arteries (bend arrow), bile ducts(arrow head) and portal vein] are normal (original magnification ×100). D: Section of livers from treatment group (APAP+ 200 mg/kg b.w essential oil) without of any necrotic zone. Bigger sinusoids (arrow), smaller and more basophilic hepatocytes were observed. The histological structures are relatively disarray (D 1 : original magnification ×40, D 2 :×100). E: Section of liver from positive control group (BHT (10 mg/kg b.w)) without any necrotic zone, sinusoids (arrows) and hepatocytes around of central vein are normal (star) (original magnification ×100).