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Table 1 Sequences of adaptors and primers for methylation-sensitive amplification polymorphism analysis

From: Correlation between DNA methylation and chronological age of Moso bamboo (Phyllostachys heterocycla var. pubescens)

Adaptors and primers EcoRI(E) HpaII/MspI(HM)
Adaptor 1 5'-CTCGTAGACTGCGTACC-3' 5'-GACGATGAGTCCTGAG-3'
Adaptor 2 5'-AATTGGTACGCAGTC-3' 5'-CGCTCAGGACTCAT-3'
Amplification primer 5'-GACTGCGTACCAATTC-3' 5'-GATGAGTCCTGAGCGG-3'
Selective 5'-GACTGCGTACCAATTCCA-3'(E3) 5'-GATGAGTCCTGAGCGGCAC-3'(HM2)
Amplification 5'-GACTGCGTACCAATTCAG-3'(E4) 5'-GATGAGTCCTGAGCGGCTA-3'(HM5)
Primer 5'-GACTGCGTACCAATTCAA-3'(E5) 5'-GATGAGTCCTGAGCGGCTC-3'(HM6)
   5'-GATGAGTCCTGAGCGGCTG-3'(HM7)