Table 1 Sequences of adaptors and primers for methylation-sensitive amplification polymorphism analysis
Adaptors and primers | EcoRI(E) | HpaII/MspI(HM) |
|---|---|---|
Adaptor 1 | 5'-CTCGTAGACTGCGTACC-3' | 5'-GACGATGAGTCCTGAG-3' |
Adaptor 2 | 5'-AATTGGTACGCAGTC-3' | 5'-CGCTCAGGACTCAT-3' |
Amplification primer | 5'-GACTGCGTACCAATTC-3' | 5'-GATGAGTCCTGAGCGG-3' |
Selective | 5'-GACTGCGTACCAATTCCA-3'(E3) | 5'-GATGAGTCCTGAGCGGCAC-3'(HM2) |
Amplification | 5'-GACTGCGTACCAATTCAG-3'(E4) | 5'-GATGAGTCCTGAGCGGCTA-3'(HM5) |
Primer | 5'-GACTGCGTACCAATTCAA-3'(E5) | 5'-GATGAGTCCTGAGCGGCTC-3'(HM6) |
5'-GATGAGTCCTGAGCGGCTG-3'(HM7) |