The seed coat development of Cyrtosia javanica . (A) The seed coat is composed of the inner (two cells thick) and outer (four cells thick) seed coats. At the zygote stage, the cell wall of the outer most layer of outer seed coat is thickening (arrow). (B) At the early globular stage, the cell wall of the outer most layer of outer seed coat has thickened (arrow). (C) At maturity, both inner and outer seed coats have dehydrated and compressed that enveloped the embryo (E) tightly. Abbreviations: IS = inner seed coat; OS = outer seed coat; Z = zygote. Scale bar = 100 μm.