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Table 1 Influence of BA and Kin on induction of multiple shoots in seedling-derived shoot tip explants of G. tenuifolia

From: In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

Cytokinin* NAA No. of explants cultured Shoot length (mm)** Explants induced multiple Shoots (%)** Average No. of shoots / explant** Explants induced callus (%)** Fresh weight/shoot (mg)**
(mg/L) (mg/L)
0 0 30 26.7 ± 2.1 cd 53.3 ± 9.1 bc 1.8 ± 0.3 c 0.0 ± 0.0 d 128.0 ± 16.7 d
0 0.1 30 40.8 ± 2.2 a 50.0 ± 9.1 bc 1.8 ± 0.2 c 56.7 ± 9.0 b 379.5 ± 33.4 c
BA 0.1 0.1 30 24.4 ± 1.3 d 93.3 ± 4.6 a 4.1 ± 0.4 b 33.3 ± 8.6 c 364.1 ± 43.6 c
BA 0.5 0.1 30 24.2 ± 1.4 d 100.0 ± 0.0 a 7.3 ± 0.6 a 13.3 ± 6.2 d 666.2 ± 68.4 b
BA 1.0 0.1 30 26.2 ± 1.7cd 100.0 ± 0.0 a 7.4 ± 0.6 a 95.0 ± 4.9 a 894.9 ± 78.4 a
Kin 0.1 0.1 30 40.1 ± 3.5 a 43.3 ± 9.0 c 1.8 ± 0.2 c 50.0 ± 9.1 bc 319.8 ± 44.2 c
Kin 0.5 0.1 30 32.8 ± 2.4 bc 70.0 ± 8.4 b 2.5 ± 0.3 c 80.0 ± 7.3 a 306.7 ± 32.6 c
Kin 1.0 0.1 30 34.6 ± 2.1 ab 56.7 ± 9.0 bc 2.3 ± 0.3 c 100.0 ± 0.0 a 253.0 ± 26.0cd
  1. *Basal medium: ½X MS basal medium supplemented with 3% sucrose and 0.9% Difco Bacto-agar. Observations were recorded after 35 days of culture.
  2. **Means followed by the same letter are not significantly different at 5% level by LSD (least significant difference) test.