In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

Botanical Studies

Table 1 Influence of BA and Kin on induction of multiple shoots in seedling-derived shoot tip explants of G. tenuifolia

Cytokinin*	NAA	No. of explants cultured	Shoot length (mm)**	Explants induced multiple Shoots (%)**	Average No. of shoots / explant**	Explants induced callus (%)**	Fresh weight/shoot (mg)**
(mg/L)	(mg/L)	No. of explants cultured	Shoot length (mm)**	Explants induced multiple Shoots (%)**	Average No. of shoots / explant**	Explants induced callus (%)**	Fresh weight/shoot (mg)**
0	0	30	26.7 ± 2.1 cd	53.3 ± 9.1 bc	1.8 ± 0.3 c	0.0 ± 0.0 d	128.0 ± 16.7 d
0	0.1	30	40.8 ± 2.2 a	50.0 ± 9.1 bc	1.8 ± 0.2 c	56.7 ± 9.0 b	379.5 ± 33.4 c
BA 0.1	0.1	30	24.4 ± 1.3 d	93.3 ± 4.6 a	4.1 ± 0.4 b	33.3 ± 8.6 c	364.1 ± 43.6 c
BA 0.5	0.1	30	24.2 ± 1.4 d	100.0 ± 0.0 a	7.3 ± 0.6 a	13.3 ± 6.2 d	666.2 ± 68.4 b
BA 1.0	0.1	30	26.2 ± 1.7cd	100.0 ± 0.0 a	7.4 ± 0.6 a	95.0 ± 4.9 a	894.9 ± 78.4 a
Kin 0.1	0.1	30	40.1 ± 3.5 a	43.3 ± 9.0 c	1.8 ± 0.2 c	50.0 ± 9.1 bc	319.8 ± 44.2 c
Kin 0.5	0.1	30	32.8 ± 2.4 bc	70.0 ± 8.4 b	2.5 ± 0.3 c	80.0 ± 7.3 a	306.7 ± 32.6 c
Kin 1.0	0.1	30	34.6 ± 2.1 ab	56.7 ± 9.0 bc	2.3 ± 0.3 c	100.0 ± 0.0 a	253.0 ± 26.0cd

*Basal medium: ½X MS basal medium supplemented with 3% sucrose and 0.9% Difco Bacto-agar. Observations were recorded after 35 days of culture.
**Means followed by the same letter are not significantly different at 5% level by LSD (least significant difference) test.