In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

Botanical Studies

Table 3 Influence of IAA and IBA concentrations on in vitro rooting of shoots of G. tenuifolia

Auxin (mg/L) *	Concentration (mg/L)	No. of shoots rooted (%)**	Average No. of roots **	Callus formation	Plants survival in greenhouse (%)
IAA	0.5	53.3 ± 9.1 b	2.4 ± 0.4 cd	-	93.3
-	1.0	63.3 ± 8.8 b	3.3 ± 0.5 c	-	100.0
-	3.0	100.0 ± 0.0 a	6.6 ± 0.7 b	+	100.0
	5.0	93.3 ± 4.6 ab	9.5 ± 0.9 a	++	96.7
IBA	0.05	3.3 ± 3.4 d	0.1 ± 0.1 e	-	90.0
	0.1	16.7 ± 6.8 c	0.9 ± 0.3 de	-	96.7
	0.5	83.3 ± 6.8 ab	6.3 ± 0.7 b	+	96.7
	1.0	96.7 ± 3.3 ab	8.6 ± 0.7 a	+++	66.7

*Basal medium:½X MS basal medium supplemented with 3% sucrose and 0.9% Difco Bacto-agar. Observations were recorded after 35 days of culture.
**Means followed by the same letter are not significantly different at 5% level by LSD (least significant difference) test.