Table 1 Parameters of OJIP kinetics of fluorescence induction curve of wild type and three mutant strains Parachlorella kessleri cells
JIP-test parameters | Wild typePC* | PC Mut2* | PC Mut4* | |
|---|---|---|---|---|
F V /F M | Maximum quantum yield of charge separation in PS2 | 0.610 ± 0.01 | 0.607 ± 0.01 | 0.506 ± 0.01 |
V J | Relative amplitude of the O-J phase | 0.38 ± 0.1 | 0.47 ± 0.01 | 0.47 ± 0.003 |
V I | Relative amplitude of the J-I phase | 0.96 ± 0.02 | 0.96 ± 0.01 | 0.94 ± 0.004 |
Mo | Initial slope of the O-J fluorescence phase | 0.60 ± 0.04 | 0.73 ± 0.02 | 0.87 ± 0.03 |
S M | Area between the fluorescence kinetic curve (O–J–I–P) and the level of Fm normalized against the Fv value | 40.97 ± 4.24 | 38.72 ± 2.32 | 30.06 ± 1.01 |
ABS/RC | Average value of absorbed photon flows in PS2 RC (of apparent size of the active antenna in PS2) | 2.57 ± 0.2 | 2.85 ± 0.1 | 3.51 ± 0.2 |
qE | Capacity for pH-induced non-photochemical fluorescence quenching | 0.14 ± 0.01 | 0.12 ± 0.01 | 0.10 ± 0.01 |
qPQ | Capacity of the quinone pool for fluorescence quenching | 0.24 ± 0.04 | 0.20 ± 0.01 | 0.19 ± 0.02 |