Figure 5
From: Overexpression of a SNARE protein AtBS14b alters BR response in Arabidopsis
Expression patterns of BR6OX2 , CPD, DWF4 and SAUR15 in bri1-5 and AtBS14b ox plants with or without BL treatment. Seedlings were grown for 7 days before treatment with 1 μM BL for 3 hours. Black bars indicate plants without BR treatment and gray bars indicate treated with BR for 3 hours. qRT-PCR results show that expression levels of BR6OX2 (a), CPD (b) and DWF4 (c) are drastically decreased after BL treatment in WS2, while slight repression was observed in bri1-5 and AtBS14b ox plants. SAUR15 induction kinetics lower in AtBS14b ox plants than in WS2 (d). Actin was used as a reference gene and the experiments were repeated at least three times with more than 10 plants were analyzed each time (*P < 0.05, **P < 0.01, ***P <0.001 t test).