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Botanical Studies

Table 1 Influence of ventilation closure types on root/shoot growth parameters and ex vitro plant survival in G. scabra Bunge X

From: In vitro propagation of Gentiana scabra Bunge – an important medicinal plant in the Chinese system of medicines

Ventilation closure treatment code

No. of rootsZ

Root lengthZ(cm)

Shoot lengthZ(cm)

Fresh weightYZ(g)

Plant survival rateZ(%)

One-stage

2AF8wk

20.2 ± 9.0 a

4.58 ± 1.16 a

2.23 ± 0.69 a

1.09 ± 0.46 a

94.44 ± 4.30 a

2DP8wk

12.4 ± 9.3 b

3.03 ± 1.18 c

1.28 ± 0.32 c

0.77 ± 0.42 c

79.13 ± 13.93 b

3DP8wk

13.0 ± 7.7 b

3.02 ± 0.89 c

1.43 ± 0.31 bc

0.78 ± 0.47 c

89.92 ± 8.36 ab

4DP8wk

18.0 ± 10.2 a

3.78 ± 1.01 b

1.51 ± 0.40 b

0.94 ± 0.33 b

91.67 ± 6.01 ab

4H2AF8wk

15.9 ± 4.98 a

4.25 ± 0.92 b

1.93 ± 0.88 a

0.93 ± 0.45 a

91.11 ± 8.31 ab

Two-stage

2AF1wk/4DP7wk

23.0 ± 9.7 bZ

4.44 ± 1.12 cZ

1.89 ± 0.42 bZ

1.14 ± 0.32 bZ

91.67 ± 2.89 aZ

2AF2wk/4DP6wk

29.6 ± 12.0 a

5.78 ± 1.13 a

2.31 ± 0.59 a

1.29 ± 0.40 ab

90.00 ± 3.33 a

2AF3wk/4DP5wk

28.6 ± 13.5 a

5.15 ± 1.20 b

2.48 ± 0.74 a

1.23 ± 0.46 b

93.33 ± 4.67 a

2AF4wk/4DP4wk

33.7 ± 12.5 a

5.89 ± 0.92 a

2.35 ± 0.51 a

1.41 ± 0.45 a

96.25 ± 3.60 a

  1. XMedium: Half strength MSBM, 0.1 mg/L−1 NAA, 3% sucrose and 0.3% gelrite, incubation period of 8 weeks.
  2. YFresh weight of rooted shoot (plantlet).
  3. ZMeans followed by the same letter of a column are not significantly different at 5% probability (p = 0.05) level by least significant difference (LSD) test.
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