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Table 1 Influence of ventilation closure types on root/shoot growth parameters and ex vitro plant survival in G. scabra Bunge X

From: In vitro propagation of Gentiana scabra Bunge an important medicinal plant in the Chinese system of medicines

Ventilation closure treatment code No. of rootsZ Root lengthZ(cm) Shoot lengthZ(cm) Fresh weightYZ(g) Plant survival rateZ(%)
One-stage 2AF8wk 20.2 ± 9.0 a 4.58 ± 1.16 a 2.23 ± 0.69 a 1.09 ± 0.46 a 94.44 ± 4.30 a
2DP8wk 12.4 ± 9.3 b 3.03 ± 1.18 c 1.28 ± 0.32 c 0.77 ± 0.42 c 79.13 ± 13.93 b
3DP8wk 13.0 ± 7.7 b 3.02 ± 0.89 c 1.43 ± 0.31 bc 0.78 ± 0.47 c 89.92 ± 8.36 ab
4DP8wk 18.0 ± 10.2 a 3.78 ± 1.01 b 1.51 ± 0.40 b 0.94 ± 0.33 b 91.67 ± 6.01 ab
4H2AF8wk 15.9 ± 4.98 a 4.25 ± 0.92 b 1.93 ± 0.88 a 0.93 ± 0.45 a 91.11 ± 8.31 ab
Two-stage 2AF1wk/4DP7wk 23.0 ± 9.7 bZ 4.44 ± 1.12 cZ 1.89 ± 0.42 bZ 1.14 ± 0.32 bZ 91.67 ± 2.89 aZ
2AF2wk/4DP6wk 29.6 ± 12.0 a 5.78 ± 1.13 a 2.31 ± 0.59 a 1.29 ± 0.40 ab 90.00 ± 3.33 a
2AF3wk/4DP5wk 28.6 ± 13.5 a 5.15 ± 1.20 b 2.48 ± 0.74 a 1.23 ± 0.46 b 93.33 ± 4.67 a
2AF4wk/4DP4wk 33.7 ± 12.5 a 5.89 ± 0.92 a 2.35 ± 0.51 a 1.41 ± 0.45 a 96.25 ± 3.60 a
  1. XMedium: Half strength MSBM, 0.1 mg/L−1 NAA, 3% sucrose and 0.3% gelrite, incubation period of 8 weeks.
  2. YFresh weight of rooted shoot (plantlet).
  3. ZMeans followed by the same letter of a column are not significantly different at 5% probability (p = 0.05) level by least significant difference (LSD) test.