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Table 6 Karyotic nature of regenerants of protoplasts of Rhizoctonia solani AG1-IC isolate CH-1 released from six generations of protoplast regenerants

From: A new variant produced by Rhizoctonia solani AG1-IC isolate CH-1 with a new type of nuclei

Origin of protoplasts

Karyotic nature*(no.)

Total

A

B

AB

Parent

12

0

4

16

2nd generation

4

0

8

12

3rd generation

    

1

3

0

7

10

2

2

0

8

10

3

2

0

8

10

4

2

0

8

10

5

6

0

4

10

4th generation

    

1

4

0

6

10

2

2

0

8

10

3

2

0

8

10

4

4

0

6

10

5

6

0

4

10

5th generation

    

1

4

0

8

12

2

4

0

8

12

3

2

0

10

12

4

3

0

9

12

5

5

0

7

12

6th generation

    

1

4

0

8

12

2

1

0

11

12

3

1

0

11

12

4

1

0

11

12

5

0

0

12

12

Total

74

0

174

248

  1. *A, homokaryon A; B, homokaryon B; AB, heterokaryon AB.