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Table 7 Karyotic nature of regenerants of protoplasts of Rhizoctonia solani AG1-IC isolate CH-1 released from mycelial mat of six generations of protoplasts treated with enzymes 2 to 4 times

From: A new variant produced by Rhizoctonia solani AG1-IC isolate CH-1 with a new type of nuclei

Origin of protoplasts and no. of enzyme treatment

Karyotic nature*(no.)

Total

A

B

AB

Parent

    

2

1

0

18

19

3

0

0

12

12

2nd generation

    

3

0

0

4

4

4

8

0

8

16

3rd generation

    

2

0

0

20

20

4

0

0

20

20

4th generation

    

3

6

0

14

20

4

3

0

17

20

5th generation

    

3

9

0

11

20

4

13

0

7

20

6th generation

    

2

7

0

13

20

4

3

0

17

20

Total

50

0

161

211

  1. *A, homokaryon A; B, homokaryon B; AB, heterokaryon AB.