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Table 7 Karyotic nature of regenerants of protoplasts of Rhizoctonia solani AG1-IC isolate CH-1 released from mycelial mat of six generations of protoplasts treated with enzymes 2 to 4 times

From: A new variant produced by Rhizoctonia solani AG1-IC isolate CH-1 with a new type of nuclei

Origin of protoplasts and no. of enzyme treatment Karyotic nature*(no.) Total
A B AB
Parent     
2 1 0 18 19
3 0 0 12 12
2nd generation     
3 0 0 4 4
4 8 0 8 16
3rd generation     
2 0 0 20 20
4 0 0 20 20
4th generation     
3 6 0 14 20
4 3 0 17 20
5th generation     
3 9 0 11 20
4 13 0 7 20
6th generation     
2 7 0 13 20
4 3 0 17 20
Total 50 0 161 211
  1. *A, homokaryon A; B, homokaryon B; AB, heterokaryon AB.