Table 3 Summary of general information for the 28 microsatellite loci isolated from Gastrodia flavilabella
Locus | Repeat motif | Primer sequence (5′-3′) | Allele size (bps) | Ta(°C) | Genbank accession no. | |||
|---|---|---|---|---|---|---|---|---|
Gfl | Gel | Gja | Gco | |||||
CT3-32 | (GGA)9 | F: TAACGGGGAATGGGGAGGCG | 137–146 | 52 | - | 54 | - | LK934509 |
R: TTGCGATCCCTCCCCTGTAC | ||||||||
CT6-4 | (GA)29 | F: CAAGAATAGGTGCCAACCTC | 110–151 | 55 | - | - | - | LK934510 |
R: GTGAGTTACTAGCGTGCGGC | ||||||||
CT6-35 | (TG)84 | F: GTCTGTTCCATTTGATATTG | 250–252 | 55 | - | - | 50 | LK934511 |
R: GCAGTAATGACCTTTGTAGT | ||||||||
CT6-65 | (TGT)36 | F: CACCGAGCTTTTTGTCAATG | 247–262 | 55 | 52 | - | 51 | LK934512 |
R: GCAATAACAATAGTAGCAGC | ||||||||
CT6-90 | (TTG)7 | F: CAACCAAGACAAGACTCATG | 132 | 55 | 55 | 52 | 55 | LK934513 |
R: ACATTCTTCCCTGGATGTTC | ||||||||
CT6-99 | (CAA)7 | F: GGCATTATCCTGTTATACTC | 138 | 55 | 50 | - | 55 | LK934514 |
R: GGGCTTTCATTTGATCATGC | ||||||||
CT6-120 | (CACAG)38 | F: TAGCAGCCATAAGTAAAGCC | 316 | 55 | - | - | - | LK934515 |
R: GTCGAGGATCAAATGAATTG | ||||||||
CT6-142 | (AAC)7 | F: GTCATGCACATTCTTCCCTG | 128–131 | 55 | 55 | - | 55 | LK934516 |
R: AGACTCATGTTGTTGATCCC | ||||||||
CT-ACT-74 | (AG)29 | F: GAGGTCCAATCTAAGATTTC | 122–156 | 54 | - | - | - | LK934517 |
R: CATGATATAATTCTCACCCC | ||||||||
CT-ACT-88 | (TGA)9 | F: TAGTGGATTTGGAGTTTGAG | 101 | 54 | - | - | 51 | LK934518 |
R: CTCATCTTTGATACCTCTTC | ||||||||
CT-ACT-136 | (CT)12 | F: ATTTAGGGTCATCGAGCACC | 140–142 | 54 | 55 | 55 | 54 | LK934519 |
R: TCGGCAAGGTGTCAAGACTC | ||||||||
CT-AG-35 | (GA)12 | F: TCTTCCCGCACCTCTTCAAC | 133–137 | 52 | 55 | 55 | 55 | LK934520 |
R: TTCAGAAGCATGGCACTGGG | ||||||||
CT-AG-45 | (CTT)12 | F: CAGAAGCCAACATATCCATC | 115–121 | 50 | 54 | - | 52 | LK934521 |
R: TCTGAAATTTAGTGTAGCGG | ||||||||
CT-AG-55 | (TGCCTC)5 | F: GTGGGGAGATTACTATTACG | 108–110 | 50 | 50 | - | 55 | LK934522 |
R: AAGGAAAGGCGTAAGGATAG | ||||||||
CT-AG-85 | (TG)9 (AG)28 | F: CCCATATGTCCTTGGTCATC | 208–248 | 54 | - | - | - | LK934523 |
R: GCTTACAACTTTCTCCCTTC | ||||||||
CT-AG-88 | (AG)15 | F: ACAACCTACACTGTCTAAAG | 152 | 55 | 54 | - | 55 | LK934524 |
R: CTTTTTTTGTGTGGTCACCG | ||||||||
CT-AG-114 | (TG)13 | F: AGTGATATGATAACACCCTC | 104 | 50 | - | - | - | LK934525 |
R: TAGATCTCTAGCTTCAACTC | ||||||||
CT-AG-127 | (TC)9 | F: AAGCTTCGCTGCCCTCTTCG | 117–123 | 54 | - | - | - | LK934526 |
R: TTGGTTTCGGGCCAGAGCTG | ||||||||
CT-AG-140 | (AG)15 | F: AGTCCTGCCTTCAAGCCTTG | 120–126 | 54 | 55 | 55 | 55 | LK934527 |
R: GAAGGATTCAAGCATGGGAG | ||||||||
CT-AG-144 | (AG)18 | F: GGCGATGTCAATTCAACAAG | 113–115 | 52 | 55 | 55 | 55 | LK934528 |
R: TAACGATAGCTGCCTTCCAC | ||||||||
CT-AG-145 | (TC)14 (ACTC)3 | F: ATCTTCGTACATCTAACCCG | 140 | 54 | - | - | 55 | LK934529 |
R: AATGAGCTCGTTGCAGCTTC | ||||||||
CT-AG-157 | (TG)14 | F: TGCAGTAATAGCATTTGCAG | 120 | 56 | 55 | - | 55 | LK934530 |
R: AGGCTGCCACTGTACTTTTC | ||||||||
CT-AGAT-19 | (TC)19 | F: TACATTGATTAGGATGCCTC | 169 | 55 | 50 | - | 50 | LK934531 |
R: ACATTTGTGCCTCCTCCAAC | ||||||||
CT-AGAT-26 | (TG)88 | F: GAATGATGCTATGTGTGCTG | 295 | 55 | - | - | - | LK934532 |
R: TGCAGTAATAGCATTTGCAG | ||||||||
CT-AGAT-131 | (CCA)7 | F: TTCAATCGCTAGTAGCTCTG | 139 | 55 | - | - | 50 | LK934533 |
R: GTTGACATTTAGTGGAGAGG | ||||||||
CT-CCA-71 | (TGG)14 | F: ACATGAGTAGGAGCATCCTC | 150–156 | 50 | - | - | 50 | LK934534 |
R: TTTCTCTTCCCCACAGCTGC | ||||||||
CT-CCA-108 | (CCA)127 | F: CATGGTGGGACATAAAACTG | 489–516 | 47 | - | - | - | LK934535 |
R: GTGGTTGTAGTCATCACTCC | ||||||||
CT-CCA-137 | (CCA)6 | F: AATCTCAGAGCCTTTCCCAG | 150 | 55 | - | - | 55 | LK934536 |
R: TTGGAGGTTGCTTGTAGAGC | ||||||||