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Table 2 Mean percentage of compounds (%) in A. herba-alba essential oils

From: Essential oil variation in wild populations of Artemisia saharae (Asteraceae) from Tunisia: chemical composition, antibacterial and antioxidant properties

Noa Compounds RIb 1c 2c 3c 4c 5c 6c 7c p1Fisher test p2Kruskal- Wallis test
1 cis-Salvene 848 0.1      0.1 0.1 na ns
2 Tricyclene 919 0.3   0.1 0.1 0.1 0.1 0.1 ns ns
3 α-Thujene 924 0.2 0.4 0.6    0.1 0.1 na ns
4 α-Pinene 930 1.6 1.9 3.2 1.9 2.7 1.4 1.7 ns ns
5 Camphenee 946 2.8 0.4 0.8 0.8 0.8 0.5 0.9 * ns
6 Verbenene 950 0.3 0.3 0.3 0.2 0.3 0.2 0.4 ns ns
7 2(5H)-Furanone, 5,5-dimethyl- 952        0.1 na ns
8 Sabinene 970 1.2 0.6 0.3 0.3 0.3 0.9 0.7 ns ns
9 3-Octenol 972    0.2      na ns
10 β-Pinenee 973 0.3   0.1   0.1   0.1 na *
11 Myrcenee 986 0.2 0.4 0.5 0.5 0.4 0.6 0.9 * *
12 Benzene,1,2,4-trimethyl- 990 0.3 0.3 0.4 0.8 0.3 0.4 0.6 ns ns
13 α-Phellandrene 1000    0.1 0.1 0.1 0.1 0.1 na ns
14 o-Isopropenyltoluene 1009 0.1 0.1 0.3 0.2 0.1 0.1 0.1 ns ns
15 α-Terpinene 1013 0.1 0.2 0.1 0.1 0.2 0.3 0.3 ns ns
16 p-Cymenee 1021 2.6 1.3 1.3 1.8 1.5 1.6 2.1 ns *
17 1,8-Cineole 1028 4.1 2.5 1.6 2.3 3.5 1.5 1.9 ns ns
18 γ-Vinyl-γ-valerolactone 1038 0.2 0.1 0.1 0.2 0.2 0.2 0.4 ns ns
19 cis-Arbusculone 1049 0.7 3.2 3.0 2.2 3.0 1.6 2.8 ns ns
20 γ-Terpinene 1055 0.2 0.4 0.2 0.3 0.3 0.4 0.5 ns ns
21 trans-Arbusculone 1067 0.6 2.4 2.1 1.6 2.1 1.3 2.2 ns ns
22 Terpinolene 1085 0.1 0.2 0.3 0.3 0.3 0.2 0.3 ns ns
23 Linalool 1095   0.1 0.1 0.2 0.1   0.1 na ns
24 Filifolone 1101 1.2 1.0 2.2 2.4 1.9 1.0 1.6 ns ns
25 α-Thujone d 1102 13.0 11.4 0.5 2.1 2.6 20.2 8.2 ns ns
26 β-Thujone d 1117 9.2 4.7 1.3 1.9 2.7 9.9 3.5 ns ns
27 cis-2-p-Menthen-1-ol 1119   0.1       na ns
28 Chrysanthenone d 1124 4.7 7.7 14.0 14.0 9.9 8.3 10.9 ns ns
29 trans-2-p-Menthen-1-ol 1135   0.1       na ns
30 1-Terpineol 1138 0.7 1.5   0.8   0.1 0.1 na ns
31 Pinocarveole 1139   1.0 5.8 2.7 4.3 3.0 3.1 *** **
32 Camphor d,e 1147 10.7 0.7 1.2 0.4 0.7 0.6 3.4 ** **
33 Sabina ketone 1158 0.3 0.1 1.9 1.0 1.2 0.8 0.4 ns ns
34 Pinocarvone 1163 1.3 1.2 1.1 0.9 1.3 0.3 1.2 ns ns
35 Borneol 1167 1.4 0.5 0.8 0.6 0.7 0.4 0.8 ns ns
36 4-Terpineol 1177 1.5 0.9 0.7 0.6 0.8 1.0 1.0 ns ns
37 p-Cymen-8-ol 1183 0.4 0.2 0.4 0.3 0.3 0.3 0.4 ns ns
38 α-Terpineole 1189 0.3 0.1       na *
39 Myrtenol 1194 0.4 0.2 0.5 0.1 0.4 0.5 0.5 ns ns
40 Verbenone 1208 0.3 0.4 0.6 0.8 0.4 0.4 0.8 ns ns
41 Carveol 1217   0.2 0.2 0.3 0.2 0.3 0.2 ns ns
42 Nordavanone 1225 0.7 0.5 0.1 1.4 0.4 0.2 0.4 ns ns
43 Cuminal 1237 0.4 0.2 0.1 0.1 0.2 0.3 0.3 ns ns
44 Piperitone 1252 0.3 0.3 0.4 0.3 0.2 0.6 0.5 ns ns
45 Chrysanthenyl acetate d,e 1257 10.2 7.9 21.1 11.5 18.7 9.1 8.2 * *
46 Isopiperitenone 1270 0.4 0.2 0.3 0.5 0.3 0.2 0.4 ns ns
47 Bornyl acetate 1281 1.4 0.2 0.4 0.2 0.5 0.2 0.5 ns ns
48 Sabinyl acetate d 1290 7.7 10.8 2.8 4.0 6.2 10.3 10.6 ns ns
49 Thymol 1301 0.5 0.3 0.6 0.4 0.3 0.6 0.6 ns ns
50 Myrtenyl acetate 1318 0.7 0.6 0.7 1.2 0.9 1.1 1.9 ns ns
51 Piperitenone 1344 0.1 0.6 0.1 0.7 0.2 0.6 1.0 ns ns
52 Norchrysanthemic acid methyl ester 1359 0.5 1.1 2.6 2.8 2.0 1.9 2.0 ns ns
53 α-Copaene 1372 2.4 0.4 0.4 0.2 0.2 0.4 0.7 ns ns
54 Methyl cinnamate 1379        0.3 na ns
55 cis-Jasmone 1395 3.5 3.4 2.9 3.3 2.2 2.2 2.8 ns ns
56 Davana furan 1399   0.1   0.2 0.1 0.1 0.1 na ns
57 β-Caryophyllene 1417 0.2 0.4 0.4 0.3 0.5 0.1 0.4 ns ns
58 p-Nitroanisole 1450 0.2 1.2 1.5 1.9 1.1 0.4 0.9 ns ns
59 Aromadendrene 1458 0.2 1.1 0.5 1.5 0.8 0.5 0.6 ns ns
60 α-Amorphene 1472 0.2 0.2 0.2 0.6 0.6 0.3 0.4 ns ns
61 Germacrene-De 1478 0.8 2.2 1.4 2.4 2.2 1.8 1.4 ** *
62 Bicyclogermacrenee 1493 0.4 1.7 0.7 1.3 1.1 0.9 0.9 *** *
63 Davana ether (isomer)e 1496 0.1 1.0 0.6 1.5 1.0 0.4 0.4 ** *
64 Davana ether (isomer)e 1504 0.2 2.5 0.7 3.4 2.1 0.9 0.7 * *
65 Davana ether (isomer) 1514   0.9 1.2 0.9 0.6 0.3 0.2 na ns
66 δ-Cadinene 1518 0.2 0.4   0.4 0.6 0.2 0.1 ns ns
67 Nerolidol 1556 0.4 0.2   0.1 0.4 0.1 0.3 na ns
68 Palustrol 1567   0.2 0.1      na ns
69 Spathulenole 1579 2.4 3.0 3.4 3.0 3.0 1.6 1.8 * *
70 Caryophyllene oxide 1588 0.4 0.9 1.1 0.8 0.9 0.4 0.7 ns ns
71 Viridiflorol 1593 0.2 1.0 0.3 0.4 0.2   0.1 na ns
72 cis-Davanonee 1608 0.3 1.3 2.1 1.2 2.6 0.7 2.8 ** **
73 Isospathulenole 1637 0.2 1.7 1.2 1.4 1.3 0.5 1.1 ** *
74 t-Cadinol 1653 0.1 0.3   0.3   0.1   na ns
75 Vulgarol Be 1688   0.8   1.0 0.6 0.3 0.3 *** **
  Total identified (%)   96.7 94.4 94.8 92.0 95.8 96.0 96.0   
  Grouped components (%)           
  Monoterpene hydrocarbons   10.0 6.2 8.3 6.6 7.1 6.5 8.2   
  Oxygenated monoterpenes   71.3 56.6 60.2 52.1 59.2 72.6 64.0   
  Sesquiterpene hydrocarbons   4.3 6.1 3.5 6.3 5.4 4.0 4.5   
  Oxygenated sesquiterpenes   4.4 14.2 10.6 14.5 13.3 5.5 8.3   
  Others   6.7 11.3 12.2 12.5 10. 8 7.4 11.0   
  1. aThe numbering refers to elution order of compounds from a HP-5MS column and their percentages were obtained by FID peak-area normalization. The percentage for each population represents the average calculated on five individuals (n = 5). bRI, retention indices calculated against C7-C25 n-alkanes mixture on the HP-5MS column. cFor the detailed description of the populations (1–7) locations, see Table 1. dMajor compound in bold fond. eCompounds with a statistically significant variation among altitudinal populations. p1: p-values using Fisher test (one-way analysis of variance) applied for normally distributed variables. Fisher test was not applicable (na) for non-Normal distributed variables. p2: p-values using non-parametric Kruskal-Wallis test (one-way analysis of variance). p1 or p2 are extremely significant (***) at p ≤ 0.001, highly significant (**) at 0.001 ≤ p ≤ 0.01, significant (*) at 0.01 ≤ p ≤ 0.05 and not significant (ns) at p > 0.05.
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