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Figure 3 | Botanical Studies

Figure 3

From: Molecular cloning and characterization of a thioredoxin from Taiwanofungus camphorata

Figure 3

Expression and purification of recombinant TcTrx in Saccharomyces cerevisiae. Fifteen μL (loading buffer without mercaptoethanol and without boiling) of each fraction was loaded into each lane of the 12% SDS-PAGE followed by Coomassie Brilliant Blue R-250 staining. Lane 1, crude extract from Saccharomyces cerevisiae expressing TcTrx; 2, flow-through proteins from the Ni-NTA column; 3, wash; 4–9, TcTrx eluted from Ni-NTA column. Molecular masses (in kDa) of standards are shown at left.

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