Figure 3
From: Molecular cloning and characterization of a thioredoxin from Taiwanofungus camphorata
Expression and purification of recombinant TcTrx in Saccharomyces cerevisiae. Fifteen μL (loading buffer without mercaptoethanol and without boiling) of each fraction was loaded into each lane of the 12% SDS-PAGE followed by Coomassie Brilliant Blue R-250 staining. Lane 1, crude extract from Saccharomyces cerevisiae expressing TcTrx; 2, flow-through proteins from the Ni-NTA column; 3, wash; 4–9, TcTrx eluted from Ni-NTA column. Molecular masses (in kDa) of standards are shown at left.