Effects of NADPH on the brightness of mBFP blue fluorescence. A. The leaves of wild type (WT) and transgenic Arabidopsis pBin-R-Se-mBFP (SE) line or pBin-A-Cy-mBFP (CY) line were directly infiltrated with NADPH as indicated concentration. Then the leaf tissues were imaged under fluorescence microscope with UV excitation (UV) or bright light (BL). The magnification is 400 X and the ruler is 20 μm. B. Tobacco leaves were infiltrated with Agrobacteria carrying pBin-R-Cy-mBFP construct or Agrobacteria only (Mock). The protoplasts isolated from infiltrated leaves were treated with NADPH, and subsequently were imaged under confocal microscope. BL, bright light. Red, chlorophyll auto-fluorescence; Blue, mBFP blue fluorescence. The magnification is 1,000 X and the ruler is 20 μm.