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Table 2 Influence of different elicitors on syringin and rutin content in callus of Saussurea involucrata Kar. et Kir

From: In vitro culture and production of syringin and rutin in Saussurea involucrata (Kar. et Kir.) – an endangered medicinal plant

Elicitor* Fresh weight (g)** Syringin (mg/g dw)*** Rutin (mg/g dw)
Control 1.31b 13.52a 0.29
Salicylic acid 1.65a 11.30bc 0.29
Abscisic acid 1.74a 8.75d 0.31
Phenylalanine 1.73a 10.73c 0.37
Sodium acetate 1.58a 12.85ab 0.32
Jasmonic acid 1.59a 14.79a 0.32
  1. *Basal medium:1/2X MSBM, BA (0.5 mg/L−1), NAA 0.5 (mg/L−1), elicitor (3.0 mg/L−1), sucrose (3 %), Gelrite (0.4 %) and pH 5.7 ± 0.1. Cultures were incubated at 20 °C. Data were recorded after 4 weeks of culture
  2. **Callus (0.2 g) was taken as inoculum. Each treatment had 50 explants. Four random samples were analyzed in HPLC
  3. ***dw: Freeze dry weight; Means followed by the same letter (a,b,c,d) were not significantly different at 5 % level