In vitro culture and production of syringin and rutin in Saussurea involucrata (Kar. et Kir.) – an endangered medicinal plant

Botanical Studies

Table 2 Influence of different elicitors on syringin and rutin content in callus of Saussurea involucrata Kar. et Kir

Elicitor*	Fresh weight (g)**	Syringin (mg/g dw)***	Rutin (mg/g dw)
Control	1.31^b	13.52^a	0.29
Salicylic acid	1.65^a	11.30^bc	0.29
Abscisic acid	1.74^a	8.75^d	0.31
Phenylalanine	1.73^a	10.73^c	0.37
Sodium acetate	1.58^a	12.85^ab	0.32
Jasmonic acid	1.59^a	14.79^a	0.32

*Basal medium:1/2X MSBM, BA (0.5 mg/L⁻¹), NAA 0.5 (mg/L⁻¹), elicitor (3.0 mg/L⁻¹), sucrose (3 %), Gelrite (0.4 %) and pH 5.7 ± 0.1. Cultures were incubated at 20 °C. Data were recorded after 4 weeks of culture
**Callus (0.2 g) was taken as inoculum. Each treatment had 50 explants. Four random samples were analyzed in HPLC
***dw: Freeze dry weight; Means followed by the same letter (a,b,c,d) were not significantly different at 5 % level