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Botanical Studies

Table 3 Influence of PGRs and container closure on callus proliferation in Saussurea involucrata Kar. et Kir

From: In vitro culture and production of syringin and rutin in Saussurea involucrata (Kar. et Kir.) – an endangered medicinal plant

PGR (mg/L−1)*

Callus (g)

2,4-D

BA

NAA

2 Aluminum foils**

3 Dispense papers**

0

0.5

0.5

0.57b

1.04a

0.5

0.5

0

0.32e

0.64bc

1.0

1.0

0

0.46c

0.74b

0.5

1.0

0

0.44dc

0.63bc

0.5

2.0

0

0.38de

0.52c

  1. *Basal medium: 1/2X MSBM, 3 % sucrose, 0.4 % Gelrite and pH 5.7 ± 0.1. Data were recorded after 4 weeks of culture
  2. Each treatment had 30 explants in three replicates. Cultures were incubated at 20 °C. Callus (0.2 g) was taken as the initial inoculum
  3. **Means followed by the same letter (a,b,c,d,e) in a column were not significantly different at the % level by LSD test
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