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Table 3 Influence of PGRs and container closure on callus proliferation in Saussurea involucrata Kar. et Kir

From: In vitro culture and production of syringin and rutin in Saussurea involucrata (Kar. et Kir.) – an endangered medicinal plant

PGR (mg/L−1)* Callus (g)
2,4-D BA NAA 2 Aluminum foils** 3 Dispense papers**
0 0.5 0.5 0.57b 1.04a
0.5 0.5 0 0.32e 0.64bc
1.0 1.0 0 0.46c 0.74b
0.5 1.0 0 0.44dc 0.63bc
0.5 2.0 0 0.38de 0.52c
  1. *Basal medium: 1/2X MSBM, 3 % sucrose, 0.4 % Gelrite and pH 5.7 ± 0.1. Data were recorded after 4 weeks of culture
  2. Each treatment had 30 explants in three replicates. Cultures were incubated at 20 °C. Callus (0.2 g) was taken as the initial inoculum
  3. **Means followed by the same letter (a,b,c,d,e) in a column were not significantly different at the % level by LSD test