Fig. 4From: Proteomic analysis of broccoli (Brassica oleracea) under high temperature and waterlogging stressesExpression levels of Rubisco genes in stressed plants. Expression analysis of leaf Rubisco large (RubL, a) and small (RubS, b) RNA in TSS-AVRDC-2 and B-75 plants under control (C), waterlogging (F), 40 °C (H), and waterlogging at 40 °C (HF) treatments for 72 h. Total RNA was analyzed by RNA gel blotting with cDNA sequences of RubL and RubS subunits as specific gene probes. Ethidium bromide staining of 28S rRNA gel was shown as a loading control. The ratios of RubL and RubS RNA to 28S rRNA in each reaction were calculated, and the ratio of the control reaction was treated as a value of one for determining the relative ratios of the other reactionsBack to article page