Fig. 1

Molecular and cellular characterization of Arabidopsis BET11 and BET12 genes. a Diagram of BET11 gene (At3g58170); exons appear as black boxes. The T-DNA insert in mutant line bet11-1 (SAIL_509_C09) is on exon 5, the ATG start codon appears to the right, and the TGA stop codon is to the left. UTRs appear in grey. The location of the complementary primer sequences for genotyping and quantitative PCR appear as arrowheads. b Diagram of BET12 gene (At4g14455). The T-DNA insert in mutant line bet12-1 (SALK_124063) is on exon 2. c Segregation of bet11-1 and bet12-1 alleles in double mutants. Non-Mendelian segregation of the homozygous alleles is statistically significant, which suggests severe defects in transmission. d Quantification of relative expression of the 3′ end of BET11 and BET12 in the WT, double mutants and complementation lines. e Subcellular localization of BET11 and BET12 N-terminal, GFP-tagged proteins in Arabidopsis protoplasts. Both proteins showed partial colocalization with the Golgi marker Man1 (Man1:RFP). Scale bars 10 μm. Data are mean ± SD (n = 3) from three biological samples. *P < 0.1, **P < 0.01, ***P < 0.001 (Student t test) compared to the WT